Standard Curve (Sample)
(Fig.1. Human ADAM metallopeptidase with thrombospondin type 1 motif4 (ADAMTS4) Standard Curve.)
Human ADAM Metallopeptidase With Thrombospondin Type 1 Motif4 (ADAMTS4) ELISA Kit | ADAMTS4 elisa kit
Human ADAM Metallopeptidase With Thrombospondin Type 1 Motif4 (ADAMTS4) ELISA Kit
Gene Names
ADAMTS4; ADMP-1; ADAMTS-2; ADAMTS-4
Reactivity
Human
Synonyms
ADAM Metallopeptidase With Thrombospondin Type 1 Motif4 (ADAMTS4); N/A; Human ADAM Metallopeptidase With Thrombospondin Type 1 Motif4 (ADAMTS4) ELISA Kit; ADAMTS4; ADAMTS-2; ADAMTS-4; ADMP-1; KIAA0688; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif; 4; aggrecanase-1; ADAMTS4 elisa kit
Reactivity
Human
Specificity
Human ADAM metallopeptidase with thrombospondin type 1 motif4 (ADAMTS4) ELISA Kit has high sensitivity and excellent specificity for detection of Human ADAMTS4. No significant cross-reactivity or interference between Human ADAMTS4 and analogues was observed.
Sequence Length
837
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human ADAM metallopeptidase with thrombospondin type 1 motif4 (ADAMTS4) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human ADAM metallopeptidase with thrombospondin type 1 motif4 (ADAMTS4) Standard Curve.)
Related Product Information for ADAMTS4 elisa kit
Description: This Human ADAM metallopeptidase with thrombospondin type 1 motif4 (ADAMTS4) ELISA Kit employs a two-site sandwich ELISA to quantitate ADAMTS4 in samples. An antibody specific for ADAMTS4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ADAMTS4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADAMTS4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADAMTS4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: ADAMTS4 encodes a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) protein family. Members of the family share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. The enzyme encoded by this gene lacks a C-terminal TS motif. It is responsible for the degradation of aggrecan, a major proteoglycan of cartilage, and brevican, a brain-specific extracellular matrix protein. The cleavage of aggrecan and brevican suggests key roles of this enzyme in arthritic disease and in the central nervous system, potentially, in the progression of glioma.
Background: ADAMTS4 encodes a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) protein family. Members of the family share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. The enzyme encoded by this gene lacks a C-terminal TS motif. It is responsible for the degradation of aggrecan, a major proteoglycan of cartilage, and brevican, a brain-specific extracellular matrix protein. The cleavage of aggrecan and brevican suggests key roles of this enzyme in arthritic disease and in the central nervous system, potentially, in the progression of glioma.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
A disintegrin and metalloproteinase with thrombospondin motifs 4 isoform 1 preproprotein
NCBI Official Synonym Full Names
ADAM metallopeptidase with thrombospondin type 1 motif 4
NCBI Official Symbol
ADAMTS4
NCBI Official Synonym Symbols
ADMP-1; ADAMTS-2; ADAMTS-4
NCBI Protein Information
A disintegrin and metalloproteinase with thrombospondin motifs 4
UniProt Protein Name
A disintegrin and metalloproteinase with thrombospondin motifs 4
UniProt Gene Name
ADAMTS4
UniProt Synonym Gene Names
KIAA0688; ADAM-TS 4; ADAM-TS4; ADAMTS-4
UniProt Entry Name
ATS4_HUMAN
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Product Notes
The Human ADAMTS4 adamts4 (Catalog #AAA331109) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA331109 ELISA Kit recognizes Human ADAMTS4. It is sometimes possible for the material contained within the vial of "ADAM Metallopeptidase With Thrombospondin Type 1 Motif4 (ADAMTS4), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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