Human Adenylate Cyclase Activating Polypeptide 1, Pituitary (ADCYAP1) ELISA Kit | ADCYAP1 elisa kit

Human Adenylate Cyclase Activating Polypeptide 1, Pituitary (ADCYAP1) ELISA Kit

Cat. Num. AAA182272

• Gene Names: ADCYAP1; PACAP
• Reactivity: Human
• Synonyms: Adenylate Cyclase Activating Polypeptide 1, Pituitary (ADCYAP1); N/A; Human Adenylate Cyclase Activating Polypeptide 1, Pituitary (ADCYAP1) ELISA Kit; PACAP; PRP-48; Pituitary Adenylate Cyclase Activating Peptide; ADCYAP1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Adenylate Cyclase Activating Polypeptide 1, Pituitary (ADCYAP1).; No significant cross-reactivity or interference between Adenylate Cyclase Activating Polypeptide 1, Pituitary (ADCYAP1) and analogues was observed.
• Sequence Length: 176
• Assay Type: Competitive Inhibition
• Samples: Serum, Plasma, Tissue Homogenates and Other Biological Fluids
• Detection Range: 6.17-500pg/mL
• Sensitivity: < 2.55pg/mL

• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Adenylate Cyclase Activating Polypeptide 1, Pituitary (ADCYAP1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Adenylate Cyclase Activating Polypeptide 1, Pituitary (ADCYAP1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Standard Curve (Sample)

Related Product Information for ADCYAP1 elisa kit

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Adenylate Cyclase Activating Polypeptide 1, Pituitary (ADCYAP1) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Adenylate Cyclase Activating Polypeptide 1, Pituitary (ADCYAP1) and unlabeled Adenylate Cyclase Activating Polypeptide 1, Pituitary (ADCYAP1) (Standards or samples) with the pre-coated antibody specific to Adenylate Cyclase Activating Polypeptide 1, Pituitary (ADCYAP1). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Adenylate Cyclase Activating Polypeptide 1, Pituitary (ADCYAP1) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Adenylate Cyclase Activating Polypeptide 1, Pituitary (ADCYAP1) in the sample.

NCBI and Uniprot Product Information

• NCBI GI #: 119622111
• NCBI GeneID: 116
• UniProt Accession #: P18509; Q52LQ0; B2R7N4
• Molecular Weight: 18,835 Da
• NCBI Official Full Name: adenylate cyclase activating polypeptide 1 (pituitary)
• NCBI Official Synonym Full Names: adenylate cyclase activating polypeptide 1
• NCBI Official Symbol: ADCYAP1
• NCBI Official Synonym Symbols: PACAP
• NCBI Protein Information: pituitary adenylate cyclase-activating polypeptide
• UniProt Protein Name: Pituitary adenylate cyclase-activating polypeptide
• UniProt Gene Name: ADCYAP1
• UniProt Synonym Gene Names: PACAP; PACAP-27; PACAP27; PACAP-38; PACAP38