Standard Curve (Sample)
(Fig.1. Human Adenylate cyclase type 2 (ADCY2) Standard Curve.)
Human Adenylate Cyclase Type 2 (ADCY2) ELISA Kit | ADCY2 elisa kit
Human Adenylate Cyclase Type 2 (ADCY2) ELISA Kit
Gene Names
ADCY2; AC2; HBAC2
Reactivity
Human
Synonyms
Adenylate Cyclase Type 2 (ADCY2); N/A; Human Adenylate Cyclase Type 2 (ADCY2) ELISA Kit; ADCY2; AC2; FLJ16822; FLJ45092; HBAC2; KIAA1060; MGC133314; 3; 5-cyclic AMP synthetase; ATP pyrophosphate-lyase; adenylate cyclase 2; adenylate cyclase; adenylyl cyclase 2; type adenylate cyclase; ADCY2 elisa kit
Reactivity
Human
Specificity
Human Adenylate cyclase type 2 (ADCY2) ELISA Kit has high sensitivity and excellent specificity for detection of Human ADCY2. No significant cross-reactivity or interference between Human ADCY2 and analogues was observed.
Sequence Length
1091
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human Adenylate cyclase type 2 (ADCY2) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human Adenylate cyclase type 2 (ADCY2) Standard Curve.)
Related Product Information for ADCY2 elisa kit
Description: This Human Adenylate cyclase type 2 (ADCY2) ELISA Kit employs a two-site sandwich ELISA to quantitate ADCY2 in samples. An antibody specific for ADCY2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ADCY2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADCY2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADCY2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Adenylate cyclase type 2 is a member of the family of adenylate cyclases, which are membrane-associated enzymes that catalyze the formation of the secondary messenger cyclic adenosine monophosphate (cAMP). This enzyme is insensitive to Ca(2+)/calmodulin, and is stimulated by the G protein beta and gamma subunit complex. The amino acid sequence of ADCY2 displayed significant homology with ADCY8 in the highly conserved adenylyl cyclase domain (250 amino acids) found in the 3-prime cytoplasmic portion of all mammalian adenylyl cyclases. However, outside this domain, the homology was extremely low.Semiquantitative RT-PCR detected ADCY2 in all tissues examined except peripheral blood leukocytes. Highest expression was detected in brain, skeletal muscle, and testis.
Background: Adenylate cyclase type 2 is a member of the family of adenylate cyclases, which are membrane-associated enzymes that catalyze the formation of the secondary messenger cyclic adenosine monophosphate (cAMP). This enzyme is insensitive to Ca(2+)/calmodulin, and is stimulated by the G protein beta and gamma subunit complex. The amino acid sequence of ADCY2 displayed significant homology with ADCY8 in the highly conserved adenylyl cyclase domain (250 amino acids) found in the 3-prime cytoplasmic portion of all mammalian adenylyl cyclases. However, outside this domain, the homology was extremely low.Semiquantitative RT-PCR detected ADCY2 in all tissues examined except peripheral blood leukocytes. Highest expression was detected in brain, skeletal muscle, and testis.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
adenylate cyclase type 2
NCBI Official Synonym Full Names
adenylate cyclase 2
NCBI Official Symbol
ADCY2
NCBI Official Synonym Symbols
AC2; HBAC2
NCBI Protein Information
adenylate cyclase type 2
UniProt Protein Name
Adenylate cyclase type 2
UniProt Gene Name
ADCY2
UniProt Synonym Gene Names
KIAA1060
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Product Notes
The Human ADCY2 adcy2 (Catalog #AAA331118) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA331118 ELISA Kit recognizes Human ADCY2. It is sometimes possible for the material contained within the vial of "Adenylate Cyclase Type 2 (ADCY2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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