Human ADP ribosylation factor like protein 13B (ARL13B) ELISA Kit | ARL13B elisa kit

Human ADP ribosylation factor like protein 13B (ARL13B) ELISA Kit

Cat. Num. AAA27340

• Gene Names: ARL13B; JBTS8; ARL2L1
• Reactivity: Human
• Synonyms: ADP ribosylation factor like protein 13B (ARL13B); N/A; Human ADP ribosylation factor like protein 13B (ARL13B) ELISA Kit; ARL13B elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of ARL13B. No significant cross-reactivity or interference between ARL13B and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ARL13B and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 0.1 ng/ml

• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for ARL13B elisa kit

Principle of the Assay: ARL13B ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-ARL13B antibody and an ARL13B-HRP conjugate. The assay sample and buffer are incubated together with ARL13B-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ARL13B concentration since ARL13B from samples and ARL13B-HRP conjugate compete for the anti-ARL13B antibody binding site. Since the number of sites is limited, as more sites are occupied by ARL13B from the sample, fewer sites are left to bind ARL13B-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ARL13B concentration in each sample is interpolated from this standard curve.

Intended Uses: This ARL13B ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ARL13B. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Product Categories/Family for ARL13B elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 115503786
• NCBI GeneID: 200894
• Molecular Weight: 37,087 Da
• NCBI Official Full Name: ADP-ribosylation factor-like protein 13B
• NCBI Official Synonym Full Names: ADP-ribosylation factor-like 13B
• NCBI Official Symbol: ARL13B
• NCBI Official Synonym Symbols: JBTS8; ARL2L1
• NCBI Protein Information: ADP-ribosylation factor-like protein 13B; ARL2-like protein 1; ADP-ribosylation factor-like 2-like 1
• UniProt Protein Name: ADP-ribosylation factor-like protein 13B
• UniProt Gene Name: ARL13B
• UniProt Synonym Gene Names: ARL2L1; ARL2-like protein 1
• UniProt Entry Name: AR13B_HUMAN