Human AFP-L3 ELISA Kit | AFP-L3 elisa kit
Human AFP-L3 (Alpha-Fetoprotein Lens Culinaris Agglutinin 3) ELISA Kit
Reactivity
Human
Synonyms
AFP-L3; N/A; Human AFP-L3 (Alpha-Fetoprotein Lens Culinaris Agglutinin 3) ELISA Kit; AFP-L3 elisa kit
Reactivity
Human
Specificity
This kit recognizes Human AFP-L3 in samples. No significant cross-reactivity or interference between Human AFP-L3 and analogues was observed.
Sequence Length
234
Samples
Serum, plasma and other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
0.63-40ng/mL
Sensitivity
0.38ng/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human AFP-L3 were tested 20 times on one plate, respectively.
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human AFP-L3 were tested on 3 different plates, 20 replicates in each plate.
Preparation and Storage
Store at 4 degree C.
Standard Curve (Sample)
Related Product Information for AFP-L3 elisa kit
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human AFP-L3 concentrations in serum, plasma and other biological fluids.
Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human AFP-L3. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human AFP-L3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human AFP-L3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human AFP-L3. You can calculate the concentration of Human AFP-L3 in the samples by comparing the OD of the samples to the standard curve.
Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human AFP-L3. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human AFP-L3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human AFP-L3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human AFP-L3. You can calculate the concentration of Human AFP-L3 in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
anti-AFP single-chain antibody, partial
