Human AGC ELISA Kit | AGC elisa kit

Human AGC (Aggrecan) ELISA Kit

Cat. Num. AAA164921

• Gene Names: Acan; Agc; cmd; Agc1; Cspg1; b2b183Clo
• Reactivity: Human
• Synonyms: AGC; N/A; Human AGC (Aggrecan) ELISA Kit; AGC elisa kit

• Reactivity: Human
• Specificity: This kit recognizes Human AGC in samples. No significant cross-reactivity or interference between Human AGC and analogues was observed.
• Sequence Length: 2132
• Assay Type: Quantitative Sandwich
• Samples: Serum, plasma and other biological fluids
• Detection Range: 0.16-10ng/mL
• Sensitivity: 0.1ng/mL

• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human AGC were tested 20 times on one plate, respectively.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human AGC were tested on 3 different plates, 20 replicates in each plate.
• Preparation and Storage: Store at 4 degree C.

Standard Curve (Sample)

Related Product Information for AGC elisa kit

Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human AGC. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human AGC and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human AGC, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human AGC. You can calculate the concentration of Human AGC in the samples by comparing the OD of the samples to the standard curve.

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human AGC concentrations in serum, plasma and other biological fluids.

NCBI and Uniprot Product Information

• NCBI GI #: 116875858
• NCBI GeneID: 11595
• NCBI Accession #: NP_031450.2
• NCBI GenBank Nucleotide #: NM_007424.2
• UniProt Accession #: Q61282; Q64021; E9QLS9
• Molecular Weight: 221,941 Da
• NCBI Official Full Name: aggrecan core protein
• NCBI Official Synonym Full Names: aggrecan
• NCBI Official Symbol: Acan
• NCBI Official Synonym Symbols: Agc; cmd; Agc1; Cspg1; b2b183Clo
• NCBI Protein Information: aggrecan core protein; CSPCP; aggrecan 1; cartilage-specific proteoglycan core protein
• UniProt Protein Name: Aggrecan core protein
• UniProt Gene Name: Acan
• UniProt Synonym Gene Names: Agc; Agc1; CSPCP
• UniProt Entry Name: PGCA_MOUSE