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product-image-AAA263019_SC15.jpg Standard Curve (Sample)

Human AGRP ELISA Kit | AGRP elisa kit

Human AGRP ELISA Kit

Gene Names
AGRP; ART; AGRT; ASIP2
Reactivity
Human
Synonyms
AGRP; N/A; Human AGRP ELISA Kit; AGRT; ART; Agouti-related protein; AGRP elisa kit
Ordering
Reactivity
Human
Specificity
The Human AGRP ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human AGRP proteins within the range of 7.8 pg/ml - 500 pg/ml.
Sequence Length
132
Samples
Cell Culture Supernates, Serum, Plasma, Urine
Sensitivity
<4 pg/ml
Preparation and Storage
Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.

Standard Curve (Sample)

product-image-AAA263019_SC15.jpg Standard Curve (Sample)
Related Product Information for AGRP elisa kit
Principle of the Assay: The Human AGRP ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human AGRP in Cell Culture Supernates, Serum, Plasma, Urine. This assay employs an antibody specific for Human AGRP coated on a 96-well plate. Standards and samples are pipetted into the wells and AGRP present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human AGRP antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of AGRP bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Background: Agouti-related protein, also called Agouti-related peptide (AgRP), is a neuropeptide produced in the brain by the AgRP/NPY neuron. It is only synthesised in NPY-containing cell bodies located in the ventromedial part of the arcuate nucleus in the hypothalamus. In humans, the agouti-related peptide is encoded by the AGRP gene. AgRP is co-expressed with Neuropeptide Y and works by increasing appetite and decreasing metabolism and energy expenditure. It is one of the most potent and long-lasting of appetite stimulators.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
181
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
14,440 Da
NCBI Official Full Name
agouti-related protein
NCBI Official Synonym Full Names
agouti related neuropeptide
NCBI Official Symbol
AGRP
NCBI Official Synonym Symbols
ART; AGRT; ASIP2
NCBI Protein Information
agouti-related protein
UniProt Protein Name
Agouti-related protein
UniProt Gene Name
AGRP
UniProt Synonym Gene Names
AGRT; ART
UniProt Entry Name
AGRP_HUMAN

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Product Notes

The Human AGRP agrp (Catalog #AAA263019) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA263019 ELISA Kit recognizes Human AGRP. It is sometimes possible for the material contained within the vial of "AGRP, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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