Standard Curve (Sample)
(Fig.1. Human Alcohol dehydrogenase 1B (ADH1B) Standard Curve.)
Human Alcohol Dehydrogenase 1B (ADH1B) ELISA Kit | ADH1B elisa kit
Human Alcohol Dehydrogenase 1B (ADH1B) ELISA Kit
Gene Names
ADH1B; ADH2; HEL-S-117
Reactivity
Human
Synonyms
Alcohol Dehydrogenase 1B (ADH1B); N/A; Human Alcohol Dehydrogenase 1B (ADH1B) ELISA Kit; ADH1B; ADH2; DKFZp686C06125; ADH; beta subunit; alcohol dehydrogenase 2 (class I); beta polypeptide; aldehyde reductase; class I alcohol dehydrogenase; ADH1B elisa kit
Reactivity
Human
Specificity
Human Alcohol dehydrogenase 1B (ADH1B) ELISA Kit has high sensitivity and excellent specificity for detection of Human ADH1B. No significant cross-reactivity or interference between Human ADH1B and analogues was observed.
Sequence Length
375
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human Alcohol dehydrogenase 1B (ADH1B) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human Alcohol dehydrogenase 1B (ADH1B) Standard Curve.)
Related Product Information for ADH1B elisa kit
Description: This Human Alcohol dehydrogenase 1B (ADH1B) ELISA Kit employs a two-site sandwich ELISA to quantitate ADH1B in samples. An antibody specific for ADH1B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ADH1B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADH1B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADH1B bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: ADH2 encoded by this gene is a member of the alcohol dehydrogenase family. Members of this enzyme family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. This encoded protein, consisting of several homo- and heterodimers of alpha, beta, and gamma subunits, exhibits high activity for ethanol oxidation and plays a major role in ethanol catabolism. Three genes encoding alpha, beta and gamma subunits are tandemly organized in a genomic segment as a gene cluster. Previously ADH1B was called ADH2. There are more genes in the family of alcohol and aldehyde dehydrogenase genes. These genes are now referred to as ADH1A, ADH1C, and ADH4, ADH5, ADH6 and ADH7.
Background: ADH2 encoded by this gene is a member of the alcohol dehydrogenase family. Members of this enzyme family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. This encoded protein, consisting of several homo- and heterodimers of alpha, beta, and gamma subunits, exhibits high activity for ethanol oxidation and plays a major role in ethanol catabolism. Three genes encoding alpha, beta and gamma subunits are tandemly organized in a genomic segment as a gene cluster. Previously ADH1B was called ADH2. There are more genes in the family of alcohol and aldehyde dehydrogenase genes. These genes are now referred to as ADH1A, ADH1C, and ADH4, ADH5, ADH6 and ADH7.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Alcohol dehydrogenase 1B
NCBI Official Synonym Full Names
alcohol dehydrogenase 1B (class I), beta polypeptide
NCBI Official Symbol
ADH1B
NCBI Official Synonym Symbols
ADH2; HEL-S-117
NCBI Protein Information
alcohol dehydrogenase 1B
UniProt Protein Name
Alcohol dehydrogenase 1B
UniProt Gene Name
ADH1B
UniProt Synonym Gene Names
ADH2
UniProt Entry Name
ADH1B_HUMAN
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Product Notes
The Human ADH1B adh1b (Catalog #AAA331125) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA331125 ELISA Kit recognizes Human ADH1B. It is sometimes possible for the material contained within the vial of "Alcohol Dehydrogenase 1B (ADH1B), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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