Human Alpha tubulin N acetyltransferase (C6orf134) ELISA Kit | C6orf134 elisa kit
Human Alpha tubulin N acetyltransferase (C6orf134) ELISA Kit
Reactivity
Human
Synonyms
Alpha tubulin N acetyltransferase (C6orf134); N/A; Human Alpha tubulin N acetyltransferase (C6orf134) ELISA Kit; C6orf134 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of ATNAT. No significant cross-reactivity or interference between ATNAT and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ATNAT and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
0.1 ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Related Product Information for C6orf134 elisa kit
Intended Uses: This ATNAT ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ATNAT. This ELISA kit for research use only, not for therapeutic or test applications!
Principle of the Assay: ATNAT ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-ATNAT antibody and an ATNAT-HRP conjugate. The assay sample and buffer are incubated together with ATNAT-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ATNAT concentration since ATNAT from samples and ATNAT-HRP conjugate compete for the anti-ATNAT antibody binding site. Since the number of sites is limited, as more sites are occupied by ATNAT from the sample, fewer sites are left to bind ATNAT-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ATNAT concentration in each sample is interpolated from this standard curve.
Principle of the Assay: ATNAT ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-ATNAT antibody and an ATNAT-HRP conjugate. The assay sample and buffer are incubated together with ATNAT-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ATNAT concentration since ATNAT from samples and ATNAT-HRP conjugate compete for the anti-ATNAT antibody binding site. Since the number of sites is limited, as more sites are occupied by ATNAT from the sample, fewer sites are left to bind ATNAT-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ATNAT concentration in each sample is interpolated from this standard curve.
Product Categories/Family for C6orf134 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
alpha tubulin N acetyltransferase
