Standard Curve (Sample)
Human alphaFP (Alpha-Fetoprotein) ELISA Kit | alphaFP elisa kit
QuicKey Pro Human alphaFP (Alpha-Fetoprotein) ELISA Kit
Gene Names
AFP; FETA; HPAFP
Reactivity
Human
Synonyms
alphaFP (Alpha-Fetoprotein); N/A; QuicKey Pro Human alphaFP (Alpha-Fetoprotein) ELISA Kit; AFP; Alpha-Fetoprotein; alphaFP elisa kit
Reactivity
Human
Specificity
This kit recognizes Human alphaFP in samples. No significant crossreactivity or interference between Human alphaFP and analogues was observed
Assay Type
Quantitative Sandwich
Samples
Human serum and plasma samples
Detection Range
0.78-50 ng/mL
Sensitivity
0.47 ng/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human alphaFP were tested 20 times on one plate, respectively.
Preparation and Storage
An unopened kit can be stored at 2-8? for 6 months. After test, the unused wells and reagents should be stored according to the table/manual.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for alphaFP elisa kit
Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human alphaFP. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human alphaFP are added to the micro ELISA plate wells. Human alphaFP in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human alphaFP. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The OD value is proportional to the concentration of Human alphaFP. The concentration of Human alphaFP in the samples is then determined by comparing the OD of the samples to the standard curve.
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of alphaFP concentrations in Human serum and plasma samples. Please contact tech-support for other sample type detection..
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of alphaFP concentrations in Human serum and plasma samples. Please contact tech-support for other sample type detection..
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
68,678 Da
NCBI Official Full Name
alpha-fetoprotein
NCBI Official Synonym Full Names
alpha-fetoprotein
NCBI Official Symbol
AFP
NCBI Official Synonym Symbols
FETA; HPAFP
NCBI Protein Information
alpha-fetoprotein; alpha-fetoglobulin; alpha-1-fetoprotein
UniProt Protein Name
Alpha-fetoprotein
UniProt Gene Name
AFP
UniProt Synonym Gene Names
HPAFP
UniProt Entry Name
FETA_HUMAN
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Product Notes
The Human alphaFP afp (Catalog #AAA177890) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA177890 ELISA Kit recognizes Human alphaFP. It is sometimes possible for the material contained within the vial of "alphaFP (Alpha-Fetoprotein), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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