Standard Curve (Sample)
Human AN1 type zinc finger protein 5 (ZFAND5) ELISA Kit | ZFAND5 elisa kit
Human AN1 type zinc finger protein 5 (ZFAND5) ELISA Kit
Gene Names
ZFAND5; ZA20D2; ZNF216; ZFAND5A
Reactivity
Human
Synonyms
AN1 type zinc finger protein 5 (ZFAND5); N/A; Human AN1 type zinc finger protein 5 (ZFAND5) ELISA Kit; ZFAND5 elisa kit
Reactivity
Human
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Detection Range
100-2500pg/mL
Sensitivity
1.0pg/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for ZFAND5 elisa kit
Intended Uses: This ZFAND5 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ZFAND5. This ELISA kit for research use only!
Principle of the Assay: ZFAND5 ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-ZFAND5 antibody and an ZFAND5-HRP conjugate. The assay sample and buffer are incubated together with ZFAND5-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ZFAND5 concentration since ZFAND5 from samples and ZFAND5-HRP conjugate compete for the anti-ZFAND5 antibody binding site. Since the number of sites is limited, as more sites are occupied by ZFAND5 from the sample, fewer sites are left to bind ZFAND5-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ZFAND5 concentration in each sample is interpolated from this standard curve.
Principle of the Assay: ZFAND5 ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-ZFAND5 antibody and an ZFAND5-HRP conjugate. The assay sample and buffer are incubated together with ZFAND5-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ZFAND5 concentration since ZFAND5 from samples and ZFAND5-HRP conjugate compete for the anti-ZFAND5 antibody binding site. Since the number of sites is limited, as more sites are occupied by ZFAND5 from the sample, fewer sites are left to bind ZFAND5-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ZFAND5 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for ZFAND5 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
23,132 Da
NCBI Official Full Name
AN1-type zinc finger protein 5
NCBI Official Synonym Full Names
zinc finger, AN1-type domain 5
NCBI Official Symbol
ZFAND5
NCBI Official Synonym Symbols
ZA20D2; ZNF216; ZFAND5A
NCBI Protein Information
AN1-type zinc finger protein 5; zinc finger protein 216; zinc finger, A20 domain containing 2; zinc finger A20 domain-containing protein 2
UniProt Protein Name
AN1-type zinc finger protein 5
UniProt Gene Name
ZFAND5
UniProt Synonym Gene Names
ZA20D2; ZNF216
UniProt Entry Name
ZFAN5_HUMAN
Similar Products
Product Notes
The Human ZFAND5 zfand5 (Catalog #AAA246137) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA246137 ELISA Kit recognizes Human ZFAND5. It is sometimes possible for the material contained within the vial of "AN1 type zinc finger protein 5 (ZFAND5), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.