Application Data
(This assay recognizes both recombinant and natural Human Ang2. The factors listed below were prepared at 50ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.)
Human Angiopoietin-2/Ang2 ELISA Kit | Ang2 elisa kit
Human Angiopoietin-2/Ang2 ELISA Kit
Reactivity
Human
Synonyms
Angiopoietin-2/Ang2; N/A; Human Angiopoietin-2/Ang2 ELISA Kit; Angiopoietin-2 ELISA Kit, Human; angiopoietin 2; AGPT2 ELISA Kit, Human; ANG2 ELISA Kit, Human; Ang2 elisa kit
Reactivity
Human
Specificity
For the quantitative determination of Human Angiopoietin-2/Ang2 concentration in serum and plasma.
Assay Type
Solid Phase Sandwich ELISA (quantitative)
Sample Types
Serum, Plasma
Detection Range
78.13-5000pg/mL
Sensitivity
49.41pg/mL
Conjugation
HRP
Interference
Preparations of the factors listed below at 50ng/mL in a mid-range recombinant human Ang2 control were assayed for interference. No significant interference was observed.
Materials Provided
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
Application Data
(This assay recognizes both recombinant and natural Human Ang2. The factors listed below were prepared at 50ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.)
Application Data
(This assay recognizes both recombinant and natural Human Ang2. The factors listed below were prepared at 50ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.)
Standard Curve (Sample)
(This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.)
Standard Curve (Sample)
(This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.)
Related Product Information for Ang2 elisa kit
This Angiopoietin-2 ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human Angiopoietin-2 protein in Serum, Plasma. It contains recombinant Human Angiopoietin-2, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
Background: Angiopoietin-2 (ANG 2, or ANGPT2), is a member of the ANG family, which plays an important role in angiogenesis during the development and growth of human cancers. Both ANGPT-1 and ANGPT-2 appear to bind to the tyrosine kinase receptor, Tie-2, found primarily on the luminal surface of endothelial cells. ANG-2's role in angiogenesis generally is considered as an antagonist for ANG1, inhibiting ANG1-promoted Tie2 signaling, which is critical for blood vessel maturation and stabilization. ANG-2 modulates angiogenesis in a cooperative manner with another important angiogenic factor, vascular endothelial growth factor A. Genetic studies have revealed that ANG-2 also is critical in lymphangiogenesis during development. ANG-2 has multiple physiologic effects that regulate vascular tone, hormone secretion, tissue growth and neural activity. Several reports indicate that ANG-2 can induce neovascularization in experimental systems due to the expression of different growth factors such as angiopoietin 2, vascular endothelial factor, and its receptor, fibroblast growth factor, platelet derived growth factor, transforming growth factor beta and epidermal growth factor. In addition, ANG-2 is strongly expressed in the vasculature of many tumors and it has been suggested that ANG-2 may act synergistically with other cytokines such as vascular endothelial growth factor to promote tumor-associated Angiogenesis and tumor progression.
Background: Angiopoietin-2 (ANG 2, or ANGPT2), is a member of the ANG family, which plays an important role in angiogenesis during the development and growth of human cancers. Both ANGPT-1 and ANGPT-2 appear to bind to the tyrosine kinase receptor, Tie-2, found primarily on the luminal surface of endothelial cells. ANG-2's role in angiogenesis generally is considered as an antagonist for ANG1, inhibiting ANG1-promoted Tie2 signaling, which is critical for blood vessel maturation and stabilization. ANG-2 modulates angiogenesis in a cooperative manner with another important angiogenic factor, vascular endothelial growth factor A. Genetic studies have revealed that ANG-2 also is critical in lymphangiogenesis during development. ANG-2 has multiple physiologic effects that regulate vascular tone, hormone secretion, tissue growth and neural activity. Several reports indicate that ANG-2 can induce neovascularization in experimental systems due to the expression of different growth factors such as angiopoietin 2, vascular endothelial factor, and its receptor, fibroblast growth factor, platelet derived growth factor, transforming growth factor beta and epidermal growth factor. In addition, ANG-2 is strongly expressed in the vasculature of many tumors and it has been suggested that ANG-2 may act synergistically with other cytokines such as vascular endothelial growth factor to promote tumor-associated Angiogenesis and tumor progression.
References
Thomas M, et al. (2009) The role of the Angiopoietins in vascular morphogenesis. Angiogenesis. 12(2): 125-37.
Hu B, et al. (2009) Angiopoietin-2: development of inhibitors for cancer therapy. Curr Oncol Rep. 11(2): 111-6.
Fiedler U, et al. (2006) Angiopoietins: a link between angiogenesis and inflammation. Trends Immunol. 27: 552-8.
Escobar E, et al. (2004) Angiotensin II, cell proliferation and angiogenesis regulator: biologic and therapeutic implications in cancer. Curr Vasc Pharmacol. 2(4): 385-99.
Hu B, et al. (2009) Angiopoietin-2: development of inhibitors for cancer therapy. Curr Oncol Rep. 11(2): 111-6.
Fiedler U, et al. (2006) Angiopoietins: a link between angiogenesis and inflammation. Trends Immunol. 27: 552-8.
Escobar E, et al. (2004) Angiotensin II, cell proliferation and angiogenesis regulator: biologic and therapeutic implications in cancer. Curr Vasc Pharmacol. 2(4): 385-99.
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Product Notes
The Human Ang2 (Catalog #AAA258376) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA258376 ELISA Kit recognizes Human Ang2. It is sometimes possible for the material contained within the vial of "Angiopoietin-2/Ang2, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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