Human Argininosuccinate lyase (ASL) ELISA Kit | ASL elisa kit

Human Argininosuccinate lyase (ASL) ELISA Kit

Cat. Num. AAA27299

• Reactivity: Human
• Synonyms: Argininosuccinate lyase (ASL); Human Argininosuccinate lyase (ASL) ELISA Kit; ASL elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of ASL. No significant cross-reactivity or interference between ASL and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ASL and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 0.1 ng/ml

• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for ASL elisa kit

Principle of the Assay: ASL ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-ASL antibody and an ASL-HRP conjugate. The assay sample and buffer are incubated together with ASL-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ASL concentration since ASL from samples and ASL-HRP conjugate compete for the anti-ASL antibody binding site. Since the number of sites is limited, as more sites are occupied by ASL from the sample, fewer sites are left to bind ASL-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ASL concentration in each sample is interpolated from this standard curve.

Intended Uses: This ASL ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ASL. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

NCBI and Uniprot Product Information

• NCBI GI #: 31377525
• NCBI GeneID: 59085
• NCBI Accession #: NP_067588.2
• NCBI GenBank Nucleotide #: NM_021577.3
• Molecular Weight: 51,550 Da
• NCBI Official Full Name: argininosuccinate lyase
• NCBI Official Synonym Full Names: argininosuccinate lyase
• NCBI Official Symbol: Asl
• NCBI Protein Information: argininosuccinate lyase; ASAL; arginosuccinase
• UniProt Protein Name: Argininosuccinate lyase
• UniProt Gene Name: Asl
• UniProt Synonym Gene Names: ASAL
• UniProt Entry Name: ARLY_RAT