Standard Curve (Sample)
Human ATP binding cassette sub family A member 12 (ABCA12) ELISA Kit | ABCA12 elisa kit
Human ATP binding cassette sub family A member 12 (ABCA12) ELISA Kit
Gene Names
ABCA12; LI2; ICR2B; ARCI4A; ARCI4B
Reactivity
Human
Synonyms
ATP binding cassette sub family A member 12 (ABCA12); N/A; Human ATP binding cassette sub family A member 12 (ABCA12) ELISA Kit; ABCA12 elisa kit
Reactivity
Human
Samples
Serum, Plasma, Cell Culture Supernatants, Body Fluid And Tissue Homogenate
Assay Type
Quantitative Competitive
Sensitivity
1.0 ng/mL.
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for ABCA12 elisa kit
Intended Uses: This AASS ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human AASS. This ELISA kit for research use only!
Principle of the Assay: AASS ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-AASS antibody and an AASS-HRP conjugate. The assay sample and buffer are incubated together with AASS-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AASS concentration since AASS from samples and AASS-HRP conjugate compete for the anti-AASS antibody binding site. Since the number of sites is limited, as more sites are occupied by AASS from the sample, fewer sites are left to bind AASS-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AASS concentration in each sample is interpolated from this standard curve.
Principle of the Assay: AASS ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-AASS antibody and an AASS-HRP conjugate. The assay sample and buffer are incubated together with AASS-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AASS concentration since AASS from samples and AASS-HRP conjugate compete for the anti-AASS antibody binding site. Since the number of sites is limited, as more sites are occupied by AASS from the sample, fewer sites are left to bind AASS-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AASS concentration in each sample is interpolated from this standard curve.
Product Categories/Family for ABCA12 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
256,960 Da
NCBI Official Full Name
ATP-binding cassette sub-family A member 12
NCBI Official Synonym Full Names
ATP-binding cassette, sub-family A (ABC1), member 12
NCBI Official Symbol
ABCA12
NCBI Official Synonym Symbols
LI2; ICR2B; ARCI4A; ARCI4B
NCBI Protein Information
ATP-binding cassette sub-family A member 12; ATP-binding cassette transporter 12
UniProt Protein Name
ATP-binding cassette sub-family A member 12
UniProt Gene Name
ABCA12
UniProt Synonym Gene Names
ABC12; ATP-binding cassette 12
UniProt Entry Name
ABCAC_HUMAN
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Product Notes
The Human ABCA12 abca12 (Catalog #AAA244740) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA244740 ELISA Kit recognizes Human ABCA12. It is sometimes possible for the material contained within the vial of "ATP binding cassette sub family A member 12 (ABCA12), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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