Standard Curve (Sample)
(Fig.1. Human ATP-dependent zinc metalloprotease YME1L1 (YME1L1) Standard Curve.)
Human ATP-Dependent Zinc Metalloprotease YME1L1 (YME1L1) ELISA Kit | YME1L1 elisa kit
Human ATP-Dependent Zinc Metalloprotease YME1L1 (YME1L1) ELISA Kit
Gene Names
YME1L1; FTSH; MEG4; PAMP; OPA11; YME1L
Reactivity
Human
Synonyms
ATP-Dependent Zinc Metalloprotease YME1L1 (YME1L1); N/A; Human ATP-Dependent Zinc Metalloprotease YME1L1 (YME1L1) ELISA Kit; YME1L1; RP11-145E8.2; FTSH; MEG4; PAMP; YME1L; ATP-dependent metalloprotease FtsH1 homolog; YME1-like 1; YME1L1 elisa kit
Reactivity
Human
Specificity
Human ATP-dependent zinc metalloprotease YME1L1 (YME1L1) ELISA Kit has high sensitivity and excellent specificity for detection of Human YME1L1. No significant cross-reactivity or interference between Human YME1L1 and analogues was observed.
Sequence Length
773
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human ATP-dependent zinc metalloprotease YME1L1 (YME1L1) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human ATP-dependent zinc metalloprotease YME1L1 (YME1L1) Standard Curve.)
Related Product Information for YME1L1 elisa kit
Description: This Human ATP-dependent zinc metalloprotease YME1L1 (YME1L1) ELISA Kit employs a two-site sandwich ELISA to quantitate YME1L1 in samples. An antibody specific for YME1L1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any YME1L1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for YME1L1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of YME1L1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: YME1L1 is the human ortholog of yeast mitochondrial AAA metalloprotease, Yme1p. It is localized in the mitochondria and can functionally complement a yme1 disruptant yeast strain. It is proposed that this gene plays a role in mitochondrial protein metabolism and could be involved in mitochondrial pathologies. Two transcript variants encoding different isoforms have been found for this gene. YME1L1 shares 50% sequence identity with yeast Yme1, 40% identity with yeast Rca1, and 39% identity with yeast Afg3, with highest homology in the central regions of the proteins. Northern blot analysis revealed transcripts of about 2.6 and 4.4 kb in all tissues examined, with greatest abundance in adult heart, skeletal muscle, and pancreas.
Background: YME1L1 is the human ortholog of yeast mitochondrial AAA metalloprotease, Yme1p. It is localized in the mitochondria and can functionally complement a yme1 disruptant yeast strain. It is proposed that this gene plays a role in mitochondrial protein metabolism and could be involved in mitochondrial pathologies. Two transcript variants encoding different isoforms have been found for this gene. YME1L1 shares 50% sequence identity with yeast Yme1, 40% identity with yeast Rca1, and 39% identity with yeast Afg3, with highest homology in the central regions of the proteins. Northern blot analysis revealed transcripts of about 2.6 and 4.4 kb in all tissues examined, with greatest abundance in adult heart, skeletal muscle, and pancreas.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
ATP-dependent zinc metalloprotease YME1L1
NCBI Official Synonym Full Names
YME1 like 1 ATPase
NCBI Official Symbol
YME1L1
NCBI Official Synonym Symbols
FTSH; MEG4; PAMP; OPA11; YME1L
NCBI Protein Information
ATP-dependent zinc metalloprotease YME1L1
UniProt Protein Name
ATP-dependent zinc metalloprotease YME1L1
UniProt Gene Name
YME1L1
UniProt Synonym Gene Names
FTSH1; YME1L; PAMP
UniProt Entry Name
YMEL1_HUMAN
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Product Notes
The Human YME1L1 yme1l1 (Catalog #AAA330239) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA330239 ELISA Kit recognizes Human YME1L1. It is sometimes possible for the material contained within the vial of "ATP-Dependent Zinc Metalloprotease YME1L1 (YME1L1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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