Standard Curve (Sample)
Human ATP sensitive inward rectifier potassium channel 10 (KCNJ10) ELISA Kit | KCNJ10 elisa kit
Human ATP sensitive inward rectifier potassium channel 10 (KCNJ10) ELISA Kit
Gene Names
KCNJ10; KIR1.2; KIR4.1; SESAME; BIRK-10; KCNJ13-PEN
Reactivity
Human
Synonyms
ATP sensitive inward rectifier potassium channel 10 (KCNJ10); N/A; Human ATP sensitive inward rectifier potassium channel 10 (KCNJ10) ELISA Kit; KCNJ10 elisa kit
Reactivity
Human
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Detection Range
100-2500pg/mL
Sensitivity
1.0pg/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for KCNJ10 elisa kit
Intended Uses: This KCNJ10 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human KCNJ10. This ELISA kit for research use only!
Principle of the Assay||KCNJ10 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-KCNJ10 antibody and an KCNJ10-HRP conjugate. The assay sample and buffer are incubated together with KCNJ10-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the KCNJ10 concentration since KCNJ10 from samples and KCNJ10-HRP conjugate compete for the anti-KCNJ10 antibody binding site. Since the number of sites is limited, as more sites are occupied by KCNJ10 from the sample, fewer sites are left to bind KCNJ10-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The KCNJ10 concentration in each sample is interpolated from this standard curve.
Principle of the Assay||KCNJ10 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-KCNJ10 antibody and an KCNJ10-HRP conjugate. The assay sample and buffer are incubated together with KCNJ10-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the KCNJ10 concentration since KCNJ10 from samples and KCNJ10-HRP conjugate compete for the anti-KCNJ10 antibody binding site. Since the number of sites is limited, as more sites are occupied by KCNJ10 from the sample, fewer sites are left to bind KCNJ10-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The KCNJ10 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for KCNJ10 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
42,508 Da
NCBI Official Full Name
ATP-sensitive inward rectifier potassium channel 10
NCBI Official Synonym Full Names
potassium inwardly-rectifying channel, subfamily J, member 10
NCBI Official Symbol
KCNJ10
NCBI Official Synonym Symbols
KIR1.2; KIR4.1; SESAME; BIRK-10; KCNJ13-PEN
NCBI Protein Information
ATP-sensitive inward rectifier potassium channel 10; inward rectifier K+ channel KIR1.2; inward rectifier K(+) channel Kir1.2; ATP-dependent inwardly rectifying potassium channel Kir4.1; potassium channel, inwardly rectifying subfamily J member 10; glial ATP-dependent inwardly rectifying potassium channel KIR4.1
UniProt Protein Name
ATP-sensitive inward rectifier potassium channel 10
UniProt Gene Name
KCNJ10
UniProt Entry Name
KCJ10_HUMAN
Similar Products
Product Notes
The Human KCNJ10 kcnj10 (Catalog #AAA245719) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA245719 ELISA Kit recognizes Human KCNJ10. It is sometimes possible for the material contained within the vial of "ATP sensitive inward rectifier potassium channel 10 (KCNJ10), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.