Standard Curve (Sample)
Human ATPase, Ca++ Transporting, Plasma Membrane 2 (ATP2B2) ELISA Kit | ATP2B2 elisa kit
Human ATPase, Ca++ Transporting, Plasma Membrane 2 (ATP2B2) ELISA Kit
Gene Names
ATP2B2; PMCA2; PMCA2a; PMCA2i
Reactivity
Human
Synonyms
ATPase, Ca++ Transporting, Plasma Membrane 2 (ATP2B2); N/A; Human ATPase, Ca++ Transporting, Plasma Membrane 2 (ATP2B2) ELISA Kit; PMCA2; Plasma Membrane Ca2+ Pump 2; Plasma Membrane Calcium-Transporting ATPase 2; ATP2B2 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of ATP2B2. No significant cross-reactivity or interference between ATP2B2 and analogues was observed.
Sequence Length
1243
Samples
Tissue homogenates, cell lysates or other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
0.312-20ng/mL
Sensitivity
<0.112ng/mL.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ATP2B2 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ATP2B2 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for ATP2B2 elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of ATP2B2 in human tissue homogenates, cell lysates or other biological fluids.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to ATP2B2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to ATP2B2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ATP2B2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of ATP2B2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to ATP2B2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to ATP2B2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ATP2B2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of ATP2B2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
134,040 Da
NCBI Official Full Name
plasma membrane calcium-transporting ATPase 2 isoform 1
NCBI Official Synonym Full Names
ATPase plasma membrane Ca2+ transporting 2
NCBI Official Symbol
ATP2B2
NCBI Official Synonym Symbols
PMCA2; PMCA2a; PMCA2i
NCBI Protein Information
plasma membrane calcium-transporting ATPase 2
UniProt Protein Name
Plasma membrane calcium-transporting ATPase 2
UniProt Gene Name
ATP2B2
UniProt Synonym Gene Names
PMCA2; PMCA2
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Product Notes
The Human ATP2B2 atp2b2 (Catalog #AAA68512) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA68512 ELISA Kit recognizes Human ATP2B2. It is sometimes possible for the material contained within the vial of "ATPase, Ca++ Transporting, Plasma Membrane 2 (ATP2B2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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