Standard Curve (Sample)
(Fig.1. Human Autoimmune regulator (AIRE) Standard Curve.)
Human Autoimmune Regulator (AIRE) ELISA Kit | AIRE elisa kit
Human Autoimmune Regulator (AIRE) ELISA Kit
Gene Names
AIRE; APS1; APSI; PGA1; AIRE1; APECED
Reactivity
Human
Synonyms
Autoimmune Regulator (AIRE); N/A; Human Autoimmune Regulator (AIRE) ELISA Kit; AIRE; AIRE1; APECED; APS1; APSI; PGA1; OTTHUMP00000109529; autoimmune polyendocrinopathy candidiasis ectodermal dystrophy; AIRE elisa kit
Reactivity
Human
Specificity
Human Autoimmune regulator (AIRE) ELISA Kit has high sensitivity and excellent specificity for detection of Human AIRE. No significant cross-reactivity or interference between Human AIRE and analogues was observed.
Sequence Length
545
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human Autoimmune regulator (AIRE) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human Autoimmune regulator (AIRE) Standard Curve.)
Related Product Information for AIRE elisa kit
Description: This Human Autoimmune regulator (AIRE) ELISA Kit employs a two-site sandwich ELISA to quantitate AIRE in samples. An antibody specific for AIRE has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any AIRE present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AIRE is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AIRE bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: The Autoimmune Regulator is expressed in the thymus. It causes transcription of a wide selection of organ-specific genes. This reduces the threat of autoimmunity occurring by allowing the elimination of autoreactive T cells by the process of negative selection if they are too reactive to self. It is mutated in the rare autoimmune syndrome Autoimmune Polyendocrinopathy Syndrome type 1 (APS-1), also known as Autoimmune Polyendocrinopathy-Candidiasis-Ectodermal Dystrophy (APECED). Disruption of AIRE results in the development of a range of autoimmune diseases, the most common clinical conditions in the syndrome are hypoparathyroidism, primary adrenocortical failure and chronic mucocutaneous candidiasis. AIRE is expressed primarily in the thymus
Background: The Autoimmune Regulator is expressed in the thymus. It causes transcription of a wide selection of organ-specific genes. This reduces the threat of autoimmunity occurring by allowing the elimination of autoreactive T cells by the process of negative selection if they are too reactive to self. It is mutated in the rare autoimmune syndrome Autoimmune Polyendocrinopathy Syndrome type 1 (APS-1), also known as Autoimmune Polyendocrinopathy-Candidiasis-Ectodermal Dystrophy (APECED). Disruption of AIRE results in the development of a range of autoimmune diseases, the most common clinical conditions in the syndrome are hypoparathyroidism, primary adrenocortical failure and chronic mucocutaneous candidiasis. AIRE is expressed primarily in the thymus
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
autoimmune regulator
NCBI Official Synonym Full Names
autoimmune regulator
NCBI Official Symbol
AIRE
NCBI Official Synonym Symbols
APS1; APSI; PGA1; AIRE1; APECED
NCBI Protein Information
autoimmune regulator
UniProt Protein Name
Autoimmune regulator
UniProt Gene Name
AIRE
UniProt Synonym Gene Names
APECED; APECED protein
UniProt Entry Name
AIRE_HUMAN
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Product Notes
The Human AIRE aire (Catalog #AAA330846) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA330846 ELISA Kit recognizes Human AIRE. It is sometimes possible for the material contained within the vial of "Autoimmune Regulator (AIRE), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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