Human B7-1 ELISA Kit | B7-1 elisa kit

Human B7-Fc Pre-Coated ELISA Kit

Cat. Num. AAA79408

• Gene Names: CD80; B7; BB1; B7-1; B7.1; LAB7; CD28LG; CD28LG1
• Reactivity: Human
• Synonyms: B7-1; N/A; Human B7-Fc Pre-Coated ELISA Kit; B7-1 elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant Human B7-1
• Assay Type: Solid Phase Sandwich ELISA: 96-Well Strip Plate
• Detection Range: 62.5pg/ml-4000pg/ml
• Sensitivity: <10pg/ml
• Components: Anti-Human B7-1 Pre-coated 96-well strip microplate; Human B7-1 Standard; Human B7-1 Biotinylated antibody (100x); Avidin-Biotin-Peroxidase Complex (100x); Sample Diluent; Antibody Diluent; Avidin-Biotin-Peroxidase Diluent; Wash Buffer Concentrate (Powder for 1000mL); Color Developing Reagent; Stop Solution; Plate Sealers
• Interference: No detectable cross-reactivity with other relevant proteins

• Preparation and Storage: Store the unopened product at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for B7-1 elisa kit

B7-1 and B7-2 are transmembrane glycoproteins of the immunoglobulin superfamily that are expressed, along with the receptors CD28 and CTLA4, by antigen-presenting cells, and along with these receptors, constitute crucial co-stimulatory pathways for T and B cell regulatory responses. As members of the B7 family, B7-1 and B7-2 play principal roles in immunity, activating immune response and maintaining immune tolerance through engagement with CD28 and CTLA4. Co-stimulatory signals generated by B7-1 and B7-2 interactions with CD28 serve to stimulate T cell activation and prevent anergy through the amplification of T cell receptor (TCR) signaling. In contrast, interactions of the ligands with CTLA-4 serves to maintain T cell homeostasis and self-tolerance through the disruption of stimulatory signaling from B7 isoform bound CD28 complexes, and by inducing powerful inhibitory signals in T cells. B7-1 plays an important role in immune response through its amplification and regulation of T cell activity at peripheral inflammation sites. B7-1, like CTLA-4, is, however, only poorly expressed on resting dendritic cells, and its up-regulation is, therefore, considerably delayed upon immune activation. Conversely, B7-2 and CD28 are constitutively expressed by resting hematopoietic and T cells, respectively, and as a result are able to rapidly induce up-regulation upon immune activation, making them critical to the early co-stimulatory singling of immune response. Both B7-1 and B7-2 have been shown to demonstrate co-stimulatory activity in T-cell proliferation in vitro and elicit enhanced antitumor immune response in vivo.
Human B7-1 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure B7-1 with a 96-well strip plate that is pre-coated with mouse monoclonal antibody specific for B7-1. It contains NS0-expressed recombinant B7-1. The detection antibody is a biotinylated goat polyclonal antibody specific for B7-1. The kit is analytically validated with ready to use reagents.

NCBI and Uniprot Product Information

• NCBI GI #: 461606
• NCBI GeneID: 941
• UniProt Accession #: P33681; Q5DTA9; Q5DTB0
• Molecular Weight: 18,761 Da
• NCBI Official Full Name: T-lymphocyte activation antigen CD80
• NCBI Official Synonym Full Names: CD80 molecule
• NCBI Official Symbol: CD80
• NCBI Official Synonym Symbols: B7; BB1; B7-1; B7.1; LAB7; CD28LG; CD28LG1
• NCBI Protein Information: T-lymphocyte activation antigen CD80
• UniProt Protein Name: T-lymphocyte activation antigen CD80
• UniProt Gene Name: CD80
• UniProt Synonym Gene Names: CD28LG; CD28LG1; LAB7; B7