Standard Curve (Sample)
(Fig.1. Human Basic salivary proline-rich protein 2 (PRB2) Standard Curve.)
Human Basic Salivary Proline-Rich Protein 2 (PRB2) ELISA Kit | PRB2 elisa kit
Human Basic Salivary Proline-Rich Protein 2 (PRB2) ELISA Kit
Gene Names
PRB2; Ps; cP7; IB-9; PRPPRB1
Reactivity
Human
Synonyms
Basic Salivary Proline-Rich Protein 2 (PRB2); N/A; Human Basic Salivary Proline-Rich Protein 2 (PRB2) ELISA Kit; PRB2; PRPPRB1; Ps; cP7; proline-rich protein BstNI; subfamily-2 (parotid size variant); salivary proline-rich protein 2; PRB2 elisa kit
Reactivity
Human
Specificity
Human Basic salivary proline-rich protein 2 (PRB2) ELISA Kit has high sensitivity and excellent specificity for detection of Human PRB2. No significant cross-reactivity or interference between Human PRB2 and analogues was observed.
Sequence Length
416
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human Basic salivary proline-rich protein 2 (PRB2) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human Basic salivary proline-rich protein 2 (PRB2) Standard Curve.)
Related Product Information for PRB2 elisa kit
Description: This Human Basic salivary proline-rich protein 2 (PRB2) ELISA Kit employs a two-site sandwich ELISA to quantitate PRB2 in samples. An antibody specific for PRB2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PRB2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRB2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRB2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: The Ps proteins of saliva are so called because they are parotid size variants, i.e., variants in the size of parotid salivary protein. The polymorphism is determined by 1 unexpressed and 2 expressed alleles at an autosomal locus. The electrophoretic polymorphism is manifested by apparent differences in molecular weights between the Ps proteins which are glycosylated. Ps and Pm are closely linked to Pr, Pa, Db, and G1. Goodman and Karn (1983) presented evidence supporting the conclusion of Azen and Denniston (1980) that there is a molecular weight difference between the 2 allelic proteins Ps-1 and Ps-2. The difference appears to be due to an extension of the Ps-2 chain (presumably at its COOH-end). Minaguchi et al. (1988) described new variants.
Background: The Ps proteins of saliva are so called because they are parotid size variants, i.e., variants in the size of parotid salivary protein. The polymorphism is determined by 1 unexpressed and 2 expressed alleles at an autosomal locus. The electrophoretic polymorphism is manifested by apparent differences in molecular weights between the Ps proteins which are glycosylated. Ps and Pm are closely linked to Pr, Pa, Db, and G1. Goodman and Karn (1983) presented evidence supporting the conclusion of Azen and Denniston (1980) that there is a molecular weight difference between the 2 allelic proteins Ps-1 and Ps-2. The difference appears to be due to an extension of the Ps-2 chain (presumably at its COOH-end). Minaguchi et al. (1988) described new variants.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
basic salivary proline-rich protein 2 preproprotein
NCBI Official Synonym Full Names
proline rich protein BstNI subfamily 2
NCBI Official Symbol
PRB2
NCBI Official Synonym Symbols
Ps; cP7; IB-9; PRPPRB1
NCBI Protein Information
basic salivary proline-rich protein 2
UniProt Protein Name
Basic salivary proline-rich protein 2
UniProt Gene Name
PRB2
UniProt Synonym Gene Names
Salivary proline-rich protein
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Product Notes
The Human PRB2 prb2 (Catalog #AAA331368) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA331368 ELISA Kit recognizes Human PRB2. It is sometimes possible for the material contained within the vial of "Basic Salivary Proline-Rich Protein 2 (PRB2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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