Human BD-4 ELISA Kit | BD-4 elisa kit

BD-4 (Human) OmniKine ELISA Kit

Cat. Num. AAA315597

• Gene Names: DEFB104B; BD-4; hBD-4; DEFB-4
• Reactivity: Human
• Synonyms: BD-4; N/A; BD-4 (Human) OmniKine ELISA Kit; HBD-4; DEFB-4; HBD4; DEFB104B; Beta-defensin 4; BD-4 elisa kit

• Reactivity: Human
• Specificity: The OmniKine™ Human BD-4 ELISA is capable of recognizing both recombinant and naturally produced Human BD-4 proteins. The antigens listed below were tested at 50 ng/ml and did not exhibit significant cross reactivity or interference.
• Form/Format: 12 x 8-Well Microstrips
• Sequence Length: 72
• Assay Type: Quantitative Sandwich

• Preparation and Storage: Store entire kit at 4 degree C until use.

Standard Curve (Sample)

Related Product Information for BD-4 elisa kit

Principle of the Assay: The OmniKine™ Human BD-4 ELISA Kit contains the components necessary for quantitative determination of natural orrecombinant Human BD-4 concentrations within any experimental sample including cell lysates, serum and plasma. This particular immunoassay utilizes the quantitative technique of a "Sandwich" Enzyme-Linked Immunosorbent Assay (ELISA) where the target protein (antigen) isbound in a "sandwich" format by the primary capture antibodies coated to each well-bottom and the secondary detection antibodies added subsequently by the investigator. The capture antibodies coated to the bottom of each well are specific for a particular epitope on Human BD-4 while the user-added detection antibodies bind to epitopes on the captured target protein. Amid each step of the procedure, a series of wash steps must be performed to ensure the elimination of non-specific binding between proteins to other proteins or to the solid phase. After incubation and "sandwiching" of the target antigen, a peroxidase enzyme is conjugated to the constant heavy chain of the secondary antibody (either covalently or via Avidin/Streptavidin-Biotin interactions), allowing for a colorimetric reaction to ensue upon substrate addition. When the substrate TMB (3, 3', 5, 5'-Tetramethylbenzidine) is added, the reaction catalyzed by peroxidase yields a blue color that is representative of the antigen concentration. Upon sufficient color development, the reaction can be terminated through addition of Stop Solution (2 N Sulfuric Acid) where the color of the solution will turn yellow. The absorbance of each well can then be read by a spectrophotometer, allowing for generation of a standard curve and subsequent determination of protein concentration.

Background/Introduction: Human BD-4, also known as Beta-Defensin 4, is a 72 amino acid cytokine protein, encoded by the DEFB104AB gene, which is commonly known for its antimicrobial activity via synergistic effects with lysozymes and Beta-Defensin 3. The antimicrobial activity displayed by BD-4 is commonly dependent upon the sodium chloride concentration in the vicinity of the cell such that the antibiotic capability is decreased as NaCl concentration increases and vice versa. The BD-4 peptide undergoes proteolytic processing further into its mature form via cleavage of its 22 residue signal sequence, resulting in the mature 50 residue BD-4 cytokine. The mature forms of all 4 types of Beta-Defensin cytokines are distinguished from their Alpha-Defensin counterparts such that they contain the characteristic 6 cysteine structural motifs via 3 disulfide linkages. Generally, defensins form a family of microbicidal and cytotoxic peptides made by neutrophils. Moreover, members of the defensin family are highly similar in protein sequence. In terms of tissue specificity, Human BD-4 is highly expressed in male testes while expressed much less in the female uterus. Furthermore, the cytokine is highly prevalent on the plasma membranes of gastric antrum while lower in neutrophils, thyroid glands, lungs and kidneys. Aside from its antimicrobial functions, human Beta-Defensin 4 is attracted via chemical stimuli towards immature dendritic cells and memory T-cells.

NCBI and Uniprot Product Information

• NCBI GI #: 103485137
• NCBI GeneID: 503618
• NCBI Accession #: NP_001035792.1
• NCBI GenBank Nucleotide #: NM_001040702.1
• UniProt Accession #: Q8WTQ1; Q496I2; Q496I3; Q496I4
• Molecular Weight: 8,526 Da
• NCBI Official Full Name: beta-defensin 104
• NCBI Official Synonym Full Names: defensin beta 104B
• NCBI Official Symbol: DEFB104B
• NCBI Official Synonym Symbols: BD-4; hBD-4; DEFB-4
• NCBI Protein Information: beta-defensin 104
• UniProt Protein Name: Beta-defensin 104
• UniProt Gene Name: DEFB104A
• UniProt Synonym Gene Names: DEFB104; DEFB4; BD-4; DEFB-4; hBD-4