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product-image-AAA258411_SC15.jpg Standard Curve (Sample)

Human C1 inhibitor/SerpinG1 ELISA Kit | C1 elisa kit

Human C1 inhibitor/SerpinG1 ELISA Kit

Reactivity
Human
Synonyms
C1 inhibitor/SerpinG1; N/A; Human C1 inhibitor/SerpinG1 ELISA Kit; C1 inhibitor ELISA Kit, Human; serpin peptidase inhibitor, clade G (C1 inhibitor), member 1; C1IN ELISA Kit, Human; C1INH ELISA Kit, Human; C1NH ELISA Kit, Human; HAE1 ELISA Kit, Human; HAE2 ELISA Kit, Human; C1 elisa kit
Ordering
Reactivity
Human
Specificity
This assay recognizes both recombinant and natural Human SerpinG1. The factors listed below were prepared at 60 ng/mL in dilution buffer and assayed for cross -reactivity. No cross -reactivity was observed.
Assay Type
Sandwich
Samples
Serum
Detection Range
125-8000pg/mL
Sensitivity
27.4 pg/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay) - Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-assay Precision (Precision between assays) - Three samples of known concentration were tested in three separate assays to assess inter-assay precision.

Standard Curve (Sample)

product-image-AAA258411_SC15.jpg Standard Curve (Sample)
Related Product Information for C1 elisa kit
Principle of the Assay: The principle of this ELISA kit is based on theSandwich Enzyme-Linked Immunosorbent Assay (ELISA). The 96-well strip plateis precoated with a monoclonal antibody specific for Human C1 inhibitor / SerpinG1. Standards and Human C1 inhibitor / SerpinG1 present in the sample is bound by the immobilized antibody. After washing, a horseradish peroxidase conjugated anti-Human C1 inhibitor / SerpinG1antibody is added, producing an antibody-antigen-antibody "sandwich complex". Following a wash to remove any unbound antibody-enzyme reagent, a TMB substrate solution is added to the wells and color develops in proportion to the amount ofHuman C1 inhibitor / SerpinG1 bound in the initial step. The color development is stopped and the intensity of the color is measured at 450 nm.

Intended Uses: The kit applies for the accurate quantitation of Human C1 inhibitor / SerpinG1 from serum. The other biological fluids need to be validated by the end user due to the complexity of natural targets and unpredictable interference.
References
Davis AE 3rd. et al. (2004) Biological effects of C1 inhibitor. Drug News Perspect. 17(7): 439-46.
Cicardi M, et al. (2005) C1 inhibitor: molecular and clinical aspects. Springer Semin Immunopathol. 27(3): 286-98.
Wouters D, et al. (2008) C1 inhibitor: just a serine protease inhibitor. New and old considerations on therapeutic applications of C1 inhibitor. Expert Opin Biol Ther. 8(8): 1225-40.
Cugno M, et al. (2009) C1-inhibitor deficiency and angioedema: molecular mechanisms and clinical progress. Trends Mol Med. 15(2): 69-78.

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Product Notes

The Human C1 (Catalog #AAA258411) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA258411 ELISA Kit recognizes Human C1. It is sometimes possible for the material contained within the vial of "C1 inhibitor/SerpinG1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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