Human C2 calcium dependent domain containing protein 4B (FAM148B) ELISA Kit | FAM148B elisa kit

Human C2 calcium dependent domain containing protein 4B (FAM148B) ELISA Kit

Cat. Num. AAA245364

• Gene Names: C2CD4B; NLF2; FAM148B
• Reactivity: Human
• Synonyms: C2 calcium dependent domain containing protein 4B (FAM148B); N/A; Human C2 calcium dependent domain containing protein 4B (FAM148B) ELISA Kit; FAM148B elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of FAM148B. No significant cross-reactivity or interference between FAM148B and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between FAM148B and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 1.0 ng/mL

• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for FAM148B elisa kit

Principle of the Assay: FAM148B ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-FAM148B antibody and an FAM148B-HRP conjugate. The assay sample and buffer are incubated together with FAM148B-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the FAM148B concentration since FAM148B from samples and FAM148B-HRP conjugate compete for the anti-FAM148B antibody binding site. Since the number of sites is limited, as more sites are occupied by FAM148B from the sample, fewer sites are left to bind FAM148B-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FAM148B concentration in each sample is interpolated from this standard curve.

Intended Uses: This FAM148B ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human FAM148B. This ELISA kit for research use only, not for therapeutic or test applications!

NCBI and Uniprot Product Information

• NCBI GI #: 157278361
• NCBI GeneID: 388125
• NCBI Accession #: NP_001007596.2
• NCBI GenBank Nucleotide #: NM_001007595.2
• Molecular Weight: 38,769 Da
• NCBI Official Full Name: C2 calcium-dependent domain-containing protein 4B
• NCBI Official Synonym Full Names: C2 calcium-dependent domain containing 4B
• NCBI Official Symbol: C2CD4B
• NCBI Official Synonym Symbols: NLF2; FAM148B
• NCBI Protein Information: C2 calcium-dependent domain-containing protein 4B; nuclear localized factor 2; nuclear-localized factor 2; family with sequence similarity 148, member B
• UniProt Protein Name: C2 calcium-dependent domain-containing protein 4B
• UniProt Gene Name: C2CD4B
• UniProt Synonym Gene Names: FAM148B; NLF2
• UniProt Entry Name: C2C4B_HUMAN