Application Data
(This assay recognizes recombinant human CALML5. The factors listed above were prepared at 50ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.Preparations of PLK1 at 15ng/mL in a mid-range recombinant human CALML5 control were assayed for interference. No significant interference was observed.)
Human CALML5 ELISA Kit | CALML5 elisa kit
Human CALML5 ELISA Kit
Reactivity
Human
Synonyms
CALML5; N/A; Human CALML5 ELISA Kit; CALML5 ELISA Kit, Human; calmodulin like 5; CLSP ELISA Kit, Human; CALML5 elisa kit
Reactivity
Human
Specificity
The kit has been verified by high purity Human CALML5 recombinant protein.
Assay Type
Solid Phase Sandwich ELISA (quantitative)
Sample Types
Recombinant protein
Detection Range
23.44-1500pg/mL
Sensitivity
4.1pg/mL
Conjugation
HRP
Interference
Preparations of PLK1 at 15ng/mL in a mid-range recombinant human CALML5 control were assayed for interference. No significant interference was observed.
Materials Provided
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
Application Data
(This assay recognizes recombinant human CALML5. The factors listed above were prepared at 50ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.Preparations of PLK1 at 15ng/mL in a mid-range recombinant human CALML5 control were assayed for interference. No significant interference was observed.)
Application Data
(This assay recognizes recombinant human CALML5. The factors listed above were prepared at 50ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.Preparations of PLK1 at 15ng/mL in a mid-range recombinant human CALML5 control were assayed for interference. No significant interference was observed.)
Standard Curve (Sample)
(This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.)
Standard Curve (Sample)
(This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.)
Related Product Information for CALML5 elisa kit
This CALML5 ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human CALML5 protein in Recombinant protein. It contains recombinant Human CALML5, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
Background: Calmodulin-like protein 5, also known as Calmodulin-like skin protein, CALML5 and CLSP, is a protein which contains fourEF-hand domains. CALML5/CLSP is particularly abundant in the epidermis where its expression is directly related to keratinocyte differentiation.The expression is very low in lung. CALML5/CLSP binds calcium. It may be involved in terminal differentiation of keratinocytes. Coxsackievirus and adenovirus receptor (CAR) is a member of the immunoglobulin (Ig) superfamily and a component of epithelial tight junction. CAR functions as a primary receptor for coxsackievirus B and adenovirus (Ad) infection. CALML5/CLSP is closely related to CAR. The structure and dynamics of human calmodulin-like skin protein CALML5/CLSP have been characterized by NMR spectroscopy. The mobility of CALML5/CLSP has been found to be different for the N-terminal and C-terminal domains. The N-terminal domain is characterized by four stable helices, which experience large fluctuations. This is shown to be due to mutations in the hydrophobic core. The overall N-terminal domain behavior is similar both in the full-length protein and in the isolated domain.
Background: Calmodulin-like protein 5, also known as Calmodulin-like skin protein, CALML5 and CLSP, is a protein which contains fourEF-hand domains. CALML5/CLSP is particularly abundant in the epidermis where its expression is directly related to keratinocyte differentiation.The expression is very low in lung. CALML5/CLSP binds calcium. It may be involved in terminal differentiation of keratinocytes. Coxsackievirus and adenovirus receptor (CAR) is a member of the immunoglobulin (Ig) superfamily and a component of epithelial tight junction. CAR functions as a primary receptor for coxsackievirus B and adenovirus (Ad) infection. CALML5/CLSP is closely related to CAR. The structure and dynamics of human calmodulin-like skin protein CALML5/CLSP have been characterized by NMR spectroscopy. The mobility of CALML5/CLSP has been found to be different for the N-terminal and C-terminal domains. The N-terminal domain is characterized by four stable helices, which experience large fluctuations. This is shown to be due to mutations in the hydrophobic core. The overall N-terminal domain behavior is similar both in the full-length protein and in the isolated domain.
References
Mehul B., et al., 2000, J. Biol. Chem. 275:12841-12847.
Babini E., et al., 2006, Structure 14:1029-1038.
Kawabata, K. et al., 2007, Gene Ther. 14 (16):1199-207.
Babini E., et al., 2006, Structure 14:1029-1038.
Kawabata, K. et al., 2007, Gene Ther. 14 (16):1199-207.
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Product Notes
The Human CALML5 (Catalog #AAA258433) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA258433 ELISA Kit recognizes Human CALML5. It is sometimes possible for the material contained within the vial of "CALML5, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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