Human Carboxymethyl lysine, CML ELISA Kit | CML elisa kit
Human Carboxymethyl lysine, CML ELISA Kit
Reactivity
Human
Synonyms
Carboxymethyl lysine, CML; N/A; Human Carboxymethyl lysine, CML ELISA Kit; Human Carboxymethyl lysine; CML ELISA Kit; Carboxymethyl lysine; CML; CML elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of human CML. No significant cross-reactivity or interference between human CML and analogues was observed.
Assay Type
Quantitative Sandwich
Samples
Serum, plasma, tissue homogenates
Detection Range
62.5 pg/ml-4000 pg/ml
Sensitivity
< 15.6 pg/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Standard Curve (Sample)
Related Product Information for CML elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CML has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CML present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CML is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CML bound in the initial step. The color development is stopped and the intensity of the color is measured.
