Application Data
(This assay recognizes both recombinant and natural Human CPB1. The factors listed below were prepared at 50ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.)
Human Carboxypeptidase B1/CPB1 ELISA Kit | CPB1 elisa kit
Human Carboxypeptidase B1/CPB1 ELISA Kit
Reactivity
Human
Synonyms
Carboxypeptidase B1/CPB1; N/A; Human Carboxypeptidase B1/CPB1 ELISA Kit; Carboxypeptidase B1 ELISA Kit, Human; carboxypeptidase B1 (tissue); CPB ELISA Kit, Human; PASP ELISA Kit, Human; PCPB ELISA Kit, Human; CPB1 elisa kit
Reactivity
Human
Specificity
For the quantitative determination of Human Carboxypeptidase B1/CPB1 concentration in serum.
Assay Type
Solid Phase Sandwich ELISA (quantitative)
Sample Types
Serum
Detection Range
6.25-400pg/mL
Sensitivity
5.30pg/mL
Conjugation
HRP
Materials Provided
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
Application Data
(This assay recognizes both recombinant and natural Human CPB1. The factors listed below were prepared at 50ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.)
Application Data
(This assay recognizes both recombinant and natural Human CPB1. The factors listed below were prepared at 50ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.)
Standard Curve (Sample)
(This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.)
Standard Curve (Sample)
(This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.)
Related Product Information for CPB1 elisa kit
This Carboxypeptidase B1 ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human Carboxypeptidase B1 protein in Serum. It contains recombinant Human Carboxypeptidase B1, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
Background: Carboxypeptidase B1, also well known as pancreatic procarboxypeptidase B (PCPB), is a highly pancreas -specific protein (PASP), and has been identified previously as a serum marker for acute pancreatitis and pancreatic graft rejection. As the prototype for those human exopeptidases that cleave off basic C-terminal residues, CPB1 specifically cleaves the C-terminal Arg and Lys residues with a preference for Arg. The B1 and B2 forms of procarboxypeptidase B differ from each other mainly in isoelectric point.The deduced amino acid sequence of PCPB predicts a 416-amino acid preproenzyme consisting of a 15-aa signal peptide, a 95-aa activation peptide and a 37-aa mature chain. The secreted PCPB zymogen is converted to enzymatically active CPB1 by limited proteolysis by trypsin.
Background: Carboxypeptidase B1, also well known as pancreatic procarboxypeptidase B (PCPB), is a highly pancreas -specific protein (PASP), and has been identified previously as a serum marker for acute pancreatitis and pancreatic graft rejection. As the prototype for those human exopeptidases that cleave off basic C-terminal residues, CPB1 specifically cleaves the C-terminal Arg and Lys residues with a preference for Arg. The B1 and B2 forms of procarboxypeptidase B differ from each other mainly in isoelectric point.The deduced amino acid sequence of PCPB predicts a 416-amino acid preproenzyme consisting of a 15-aa signal peptide, a 95-aa activation peptide and a 37-aa mature chain. The secreted PCPB zymogen is converted to enzymatically active CPB1 by limited proteolysis by trypsin.
References
Yamamoto, K.K. et al., 1992, J. Biol. Chem. 267: 2575-2581.
Pezzilli, R. et al., 1994, Digestion. 55: 73-77.
Barbosa Pereira, P.J. et al., 2002, J. Mol. Biol. 321: 537-547.
Pezzilli, R. et al., 1994, Digestion. 55: 73-77.
Barbosa Pereira, P.J. et al., 2002, J. Mol. Biol. 321: 537-547.
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Product Notes
The Human CPB1 (Catalog #AAA258371) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA258371 ELISA Kit recognizes Human CPB1. It is sometimes possible for the material contained within the vial of "Carboxypeptidase B1/CPB1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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