Human CCL25/TECK ELISA Kit | CCL25/TECK elisa kit

Human CCL25/TECK PicoKine Quick ELISA Kit

Cat. Num. AAA128082

• Gene Names: CCL25; TECK; Ckb15; SCYA25
• Reactivity: Human
• Applications: ELISA
• Synonyms: CCL25/TECK; N/A; Human CCL25/TECK PicoKine Quick ELISA Kit; C-C motif chemokine 25; Chemokine TECK; Small-inducible cytokine A25; Thymus-expressed chemokine; CCL25; SCYA25, TECK; CCL25/TECK elisa kit

• Reactivity: Human
• Samples: cell culture supernatants, serum and plasma (heparin, EDTA)
• Assay Range: 62.5pg/ml-4000pg/ml
• Sensitivity: <10pg/ml
• Capture antibody and Detection antibody: polyclonal antibody from goat|polyclonal antibody from goat
• Anticoagulant: heparin or EDTA
• Cross Reactivity: There is no detectable cross-reactivity with other relevant proteins.

• Applicable Applications for CCL25/TECK elisa kit: ELISA
• Kit Components: Description/Quantity; Pre-coated 96-well strip microplate/1; Standard/2 vials, 10ng/tube; Biotinylated antibody (100x)/100ul; Avidin-Biotin-Peroxidase Complex (100x)/100ul; Sample Diluent/30ml; Antibody Diluent/12ml; Avidin-Biotin-Peroxidase Diluent/12ml; Color Developing Reagent (TMB)/10ml; Stop Solution/10ml; Wash Buffer (25x)/20ml; Adhesive plate sealers/4
• Materials Required But Not Provided: Microplate Reader capable of reading absorbance at 450nm.; Incubator.; Automated plate washer (optional).; Pipettes and pipette tips capable of precisely dispensing 0.5ul through 1ml volumes of aqueous solutions.; Multichannel pipettes are recommended for large amount of samples.; Deionized or distilled water.; 500ml graduated cylinders.; Test tubes for dilution.
• Sample Data:

  Concentration(pg/ml)	0	62.5	125	250	500	1,000	2,000	4,000
  O.D.	0.045	0.092	0.133	0.268	0.458	0.654	1.196	2.001

• Intra Assay Consistency & Inter Assay Consistency:

  	Intra-Assay Precision			Inter-Assay Precision
  Sample	1	2	3	1	2	3
  n	16	16	16	24	24	24
  Mean(pg/ml)	73	482	1099	107	424	1336
  Standard deviation	5.58	36.78	30.55	4.82	12.47	106.48
  CV(%)	7.6%	7.6%	2.8%	4.5%	2.9%	8%

• Reproducibility:

  Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	73	79	95	74	80	2.46	3.1%
  Sample 2	482	414	421	310	407	15.25	3.8%
  Sample 3	1099	1059	1181	1952	1323	78.7	6%

  *number of samples for each test n=16.
• Preparation and Storage: Store at 4 degree C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Standard Curve (Sample)

(Human CCL25/TECK PicoKine ELISA Kit standard curve)

Related Product Information for CCL25/TECK elisa kit

The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Categories/Family for CCL25/TECK elisa kit

Quick ELISA Kits

NCBI and Uniprot Product Information

• NCBI GI #: 212286066
• NCBI GeneID: 6370
• UniProt Accession #: O15444; Q96KJ7; A1L4J4; A6NI52; A8K9E7; B5MCA5
• Molecular Weight: 150
• NCBI Official Full Name: C-C motif chemokine 25
• NCBI Official Synonym Full Names: chemokine (C-C motif) ligand 25
• NCBI Official Symbol: CCL25
• NCBI Official Synonym Symbols: TECK; Ckb15; SCYA25
• NCBI Protein Information: C-C motif chemokine 25; TECKvar; Ck beta-15; chemokine TECK; thymus expressed chemokine; thymus-expressed chemokine; small-inducible cytokine A25; small inducible cytokine subfamily A (Cys-Cys), member 25
• UniProt Protein Name: C-C motif chemokine 25
• UniProt Gene Name: CCL25
• UniProt Synonym Gene Names: SCYA25; TECK
• UniProt Entry Name: CCL25_HUMAN