Standard Curve (Sample)
Human CCL8/MCP-2 ELISA Kit | CCL8 elisa kit
Human CCL8/MCP-2 PicoKine ELISA Kit
Gene Names
CCL8; HC14; MCP2; MCP-2; SCYA8; SCYA10
Reactivity
Human
Synonyms
CCL8/MCP-2; N/A; Human CCL8/MCP-2 PicoKine ELISA Kit; C-C motif chemokine 8; chemokine (C-C motif) ligand 8; C C motif chemokine 8; CCL 8; Ccl8; CCL8_HUMAN; Chemokine (C C motif) ligand 8; Chemokine ligand 8; HC14; MCP 2; MCP-2; MCP-2(6-76); Monocyte chemoattractant protein 2; Monocyte chemotactic protein 2; SCYA10; SCYA8; Small inducible cytokine A8; Small inducible cytokine subfamily A (Cys Cys) member 8; Small-inducible cytokine A8; CCL8 elisa kit
Reactivity
Human
Specificity
Natural and recombinant Human CCL8
Sequence Length
99
Samples
Cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)
Detection Range
15.6 pg/ml - 1,000 pg/ml
Sensitivity
<1 pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision across assays): Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for CCL8 elisa kit
Background: Chemokine (C-C motif) ligand 8, also known as monocyte chemoattractant protein 2 (MCP-2), is a protein that in humans is encoded by the CCL8 gene. CCL8 is a small cytokine belonging to the CC chemokine family. The CCL8 protein is produced as a precursor containing 109 amino acids, which is cleaved to produce mature CCL8 containing 75 amino acids. The gene for CCL8 is encoded by 3 exons and is located within a large cluster of CC chemokines on chromosome 17q11.2 in humans. Monocyte chemotactic protein 2 (MCP-2) is a CC chemokine that utilizes multiple cellular receptors to attract and activate human leukocytes.
Principle of the Assay: The Picokine™ Human CCL8 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid-phase immunoassay specially designed to measure Human CCL8 with a 96-well strip plate that is pre-coated with antibody specific for CCL8. The detection antibody is a biotinylated antibody specific for CCL8. The capture antibody is polyclonal antibody from goat and the detection antibody is polyclonal antibody from goat. The kit includes Human CCL8 protein as standards. To measure Human CCL8, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product at 450nm is linearly proportional to Human CCL8 in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human CCL8 in the sample.
Principle of the Assay: The Picokine™ Human CCL8 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid-phase immunoassay specially designed to measure Human CCL8 with a 96-well strip plate that is pre-coated with antibody specific for CCL8. The detection antibody is a biotinylated antibody specific for CCL8. The capture antibody is polyclonal antibody from goat and the detection antibody is polyclonal antibody from goat. The kit includes Human CCL8 protein as standards. To measure Human CCL8, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product at 450nm is linearly proportional to Human CCL8 in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human CCL8 in the sample.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
11,246 Da
NCBI Official Full Name
C-C motif chemokine 8
NCBI Official Synonym Full Names
chemokine (C-C motif) ligand 8
NCBI Official Symbol
CCL8
NCBI Official Synonym Symbols
HC14; MCP2; MCP-2; SCYA8; SCYA10
NCBI Protein Information
C-C motif chemokine 8; small-inducible cytokine A8; monocyte chemotactic protein 2; monocyte chemoattractant protein 2; small inducible cytokine subfamily A (Cys-Cys), member 8 (monocyte chemotactic protein 2)
UniProt Protein Name
C-C motif chemokine 8
UniProt Gene Name
CCL8
UniProt Synonym Gene Names
MCP2; SCYA10; SCYA8; MCP-2
UniProt Entry Name
CCL8_HUMAN
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Product Notes
The Human CCL8 ccl8 (Catalog #AAA11593) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA11593 ELISA Kit recognizes Human CCL8. It is sometimes possible for the material contained within the vial of "CCL8/MCP-2, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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