Human Cell death activator CIDE B (CIDEB) ELISA Kit | CIDEB elisa kit

Human Cell death activator CIDE B (CIDEB) ELISA Kit

Cat. Num. AAA245179

• Reactivity: Human
• Synonyms: Cell death activator CIDE B (CIDEB); N/A; Human Cell death activator CIDE B (CIDEB) ELISA Kit; CIDEB elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of CIDEB. No significant cross-reactivity or interference between CIDEB and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CIDEB and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 1.0 pg/mL

• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for CIDEB elisa kit

Principle of the Assay: CIDEB ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-CIDEB antibody and an CIDEB-HRP conjugate. The assay sample and buffer are incubated together with CIDEB-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CIDEB concentration since CIDEB from samples and CIDEB-HRP conjugate compete for the anti-CIDEB antibody binding site. Since the number of sites is limited, as more sites are occupied by CIDEB from the sample, fewer sites are left to bind CIDEB-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CIDEB concentration in each sample is interpolated from this standard curve.

Intended Uses: This CIDEB ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CIDEB. This ELISA kit for research use only, not for therapeutic or test applications!

NCBI and Uniprot Product Information

• NCBI GI #: 93102358
• NCBI GeneID: 27141
• NCBI Accession #: NP_055245.2
• NCBI GenBank Nucleotide #: NM_014430.2
• Molecular Weight: 24,678 Da
• NCBI Official Full Name: cell death activator CIDE-B
• NCBI Official Synonym Full Names: cell death-inducing DFFA-like effector b
• NCBI Official Symbol: CIDEB
• NCBI Protein Information: cell death activator CIDE-B
• UniProt Protein Name: Cell death activator CIDE-B
• UniProt Gene Name: CIDEB
• UniProt Entry Name: CIDEB_HUMAN