Standard Curve (Sample)
(Human CFHR5 PicoKine ELISA Kit standard curve)
Human CFHR5 ELISA Kit | CFHR5 elisa kit
Human CFHR5 PicoKine Quick ELISA Kit
Gene Names
CFHR5; FHR5; CFHL5; FHR-5; CFHR5D
Reactivity
Human
Applications
ELISA
Synonyms
CFHR5; N/A; Human CFHR5 PicoKine Quick ELISA Kit; CFHR5 elisa kit
Reactivity
Human
Specificity
Natural and recombinant Human CFHR5
Assay Type
Sandwich
Samples
Cell culture supernatants, serum and plasma (heparin, EDTA) and urine
Detection Range
0.78 ng/ml - 50 ng/ml
Sensitivity
<0.1 ng/ml
Applicable Applications for CFHR5 elisa kit
ELISA
Intra-assay Precision
Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision across assays): Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Standard Curve (Sample)
(Human CFHR5 PicoKine ELISA Kit standard curve)
Standard Curve (Sample)
(Human CFHR5 PicoKine ELISA Kit standard curve)
Related Product Information for CFHR5 elisa kit
Background: Complement factor H-related protein 5 is a protein that in humans is encoded by the CFHR5 gene. It is mapped to 1q31.3. This gene is a member of a small complement factor H (CFH) gene cluster on chromosome 1. Each member of this gene family contains multiple short consensus repeats (SCRs) typical of regulators of complement activation. The protein encoded by this gene has nine SCRs with the first two repeats having heparin binding properties, a region within repeats 5-7 having heparin binding and C reactive protein binding properties, and the C-terminal repeats being similar to a complement component 3 b (C3b) binding domain. This protein co-localizes with C3, binds C3b in a dose-dependent manner, and is recruited to tissues damaged by C-reactive protein. Allelic variations in this gene have been associated, but not causally linked, with two different forms of kidney disease: membranoproliferative glomerulonephritis type II (MPGNII) and hemolytic uraemic syndrome (HUS).
Principle of the Assay: The Quick Picokine™ Human CFHR5 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human CFHR5 in cell culture supernatants, serum and plasma (heparin, EDTA) and urine. It uses our proprietary Quick ELISA technology. Quick ELISA technology employs capture antibodies conjugated to an affinity tag that is recognized by the polyclonal antibody used to coat our Quick ELISA plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. The kit includes Human CFHR5 protein as standards. To measure Human CFHR5, add standards and samples to the wells, then add antibody cocktail. Wash the wells with PBS or TBS buffer, and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product is linearly proportional to Human CFHR5 in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human CFHR5 in the sample.
Principle of the Assay: The Quick Picokine™ Human CFHR5 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human CFHR5 in cell culture supernatants, serum and plasma (heparin, EDTA) and urine. It uses our proprietary Quick ELISA technology. Quick ELISA technology employs capture antibodies conjugated to an affinity tag that is recognized by the polyclonal antibody used to coat our Quick ELISA plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. The kit includes Human CFHR5 protein as standards. To measure Human CFHR5, add standards and samples to the wells, then add antibody cocktail. Wash the wells with PBS or TBS buffer, and add TMB. TMB is an HRP substrate and will be catalyzed to produce a blue color product, which changes into yellow after adding the acidic stop solution. The absorbance of the yellow product is linearly proportional to Human CFHR5 in the sample. Read the absorbance of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human CFHR5 in the sample.
Product Categories/Family for CFHR5 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
64,419 Da
NCBI Official Full Name
complement factor H-related protein 5
NCBI Official Synonym Full Names
complement factor H-related 5
NCBI Official Symbol
CFHR5
NCBI Official Synonym Symbols
FHR5; CFHL5; FHR-5; CFHR5D
NCBI Protein Information
complement factor H-related protein 5
UniProt Protein Name
Complement factor H-related protein 5
UniProt Gene Name
CFHR5
UniProt Synonym Gene Names
CFHL5; FHR5; FHR-5
UniProt Entry Name
FHR5_HUMAN
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Product Notes
The Human CFHR5 cfhr5 (Catalog #AAA126849) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA126849 ELISA Kit recognizes Human CFHR5. AAA Biotech's CFHR5 can be used in a range of immunoassay formats including, but not limited to, ELISA. Researchers should empirically determine the suitability of the CFHR5 cfhr5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CFHR5, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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