Standard Curve (Sample)
(Typical Standard Curve for CCK8 ELISA (Competitive))
Human Cholecystokinin 8 (CCK8) ELISA Kit | CCK8 elisa kit
Human Cholecystokinin 8 (CCK8) Mini Samples ELISA Kit
Reactivity
Human
Synonyms
Cholecystokinin 8 (CCK8); N/A; Human Cholecystokinin 8 (CCK8) Mini Samples ELISA Kit; CCK8 Mini-sample ELISA kit (Human); Sincalide; Cholecystokinin 8, Octapeptide; CCK8 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of Mini Samples Cholecystokinin 8 (CCK8). No significant cross-reactivity or interference between Mini Samples Cholecystokinin 8 (CCK8) and analogues was observed.
Reactive with Human CCK8/Cholecystokinin 8
Reactive with Human CCK8/Cholecystokinin 8
Assay Type
Competitive Inhibition
Samples
Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and other biological fluids
Detection Method
Colormetric
Featured Series
ME kit
Detection Range
2.47-200pg/mL
Sensitivity
0.87pg/mL
Sample Size
15-25uL
Assay Time
3h
Function
Detects protein (micro-volume samples 15-25 ul)
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples Cholecystokinin 8 (CCK8) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Cholecystokinin 8 (CCK8) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%, Inter-Assay: CV<12%
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Cholecystokinin 8 (CCK8) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%, Inter-Assay: CV<12%
Assay Procedure Summary
1. Prepare all reagents, samples and standards
2. Add 25ul standard or sample to each well. And then add 25uL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37 degree C
3. Aspirate and wash 3 times
4. Add 50ul prepared Detection Reagent B. Incubate 30 minutes at 37 degree C
5. Aspirate and wash 5 times
6. Add 50ul Substrate Solution. Incubate 10-20 minutes at 37 degree C
7. Add 25ul Stop Solution. Read at 450 nm immediately.
2. Add 25ul standard or sample to each well. And then add 25uL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37 degree C
3. Aspirate and wash 3 times
4. Add 50ul prepared Detection Reagent B. Incubate 30 minutes at 37 degree C
5. Aspirate and wash 5 times
6. Add 50ul Substrate Solution. Incubate 10-20 minutes at 37 degree C
7. Add 25ul Stop Solution. Read at 450 nm immediately.
Test Principle
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Mini Samples Cholecystokinin 8 (CCK8) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Mini Samples Cholecystokinin 8 (CCK8) and unlabeled Mini Samples Cholecystokinin 8 (CCK8)(Standards or samples) with the pre-coated antibody specific to Mini Samples Cholecystokinin 8 (CCK8). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Mini Samples Cholecystokinin 8 (CCK8) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Mini Samples Cholecystokinin 8 (CCK8) in the sample.
Research Area
Endocrinology, Gastroenterology, Hormone metabolism
Preparation and Storage
Store at 4 degree C for 1 month/-20 degree C for long-term (One year within shelf life)
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Standard Curve (Sample)
(Typical Standard Curve for CCK8 ELISA (Competitive))
Standard Curve (Sample)
(Typical Standard Curve for CCK8 ELISA (Competitive))
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Product Notes
The Human CCK8 (Catalog #AAA161544) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA161544 ELISA Kit recognizes Human CCK8. It is sometimes possible for the material contained within the vial of "Cholecystokinin 8 (CCK8), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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