Standard Curve (Sample)
(Fig.1. Human Claudin-8 (CLDN8) Standard Curve.)
Human Claudin-8 (CLDN8) ELISA Kit | CLDN8 elisa kit
Human Claudin-8 (CLDN8) ELISA Kit
Gene Names
CLDN8; HEL-S-79
Reactivity
Human
Synonyms
Claudin-8 (CLDN8); N/A; Human Claudin-8 (CLDN8) ELISA Kit; OTTHUMP00000101915; CLDN8 elisa kit
Reactivity
Human
Specificity
Human Claudin-8 (CLDN8) ELISA Kit has high sensitivity and excellent specificity for detection of Human CLDN8. No significant cross-reactivity or interference between Human CLDN8 and analogues was observed.
Sequence Length
225
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human Claudin-8 (CLDN8) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human Claudin-8 (CLDN8) Standard Curve.)
Related Product Information for CLDN8 elisa kit
Description: This Human Claudin-8 (CLDN8) ELISA Kit employs a two-site sandwich ELISA to quantitate CLDN8 in samples. An antibody specific for CLDN8 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CLDN8 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CLDN8 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CLDN8 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Claudins, such as CLDN8, are components of epithelial cell tight junctions. Tight junctions regulate movement of solutes and ions through the paracellular space and prevent mixing of proteins and lipids in the outer leaflet of the apical and basolateral plasma membrane domains. Cldn8 expression reduced paracellular permeability to monovalent inorganic and organic cations and to divalent cations, but not to anions or neutral substrates. The results were consistent with a model in which CLDN2 encodes a highly cation-permeable channel, whereas CLDN8 acts primarily as a cation barrier. Cldn8 was expressed primarily at the tight junction along the entire aldosterone-sensitive distal nephron and in the late segments of the thin descending limbs of long-looped nephrons.
Background: Claudins, such as CLDN8, are components of epithelial cell tight junctions. Tight junctions regulate movement of solutes and ions through the paracellular space and prevent mixing of proteins and lipids in the outer leaflet of the apical and basolateral plasma membrane domains. Cldn8 expression reduced paracellular permeability to monovalent inorganic and organic cations and to divalent cations, but not to anions or neutral substrates. The results were consistent with a model in which CLDN2 encodes a highly cation-permeable channel, whereas CLDN8 acts primarily as a cation barrier. Cldn8 was expressed primarily at the tight junction along the entire aldosterone-sensitive distal nephron and in the late segments of the thin descending limbs of long-looped nephrons.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
claudin-8
NCBI Official Synonym Full Names
claudin 8
NCBI Official Symbol
CLDN8
NCBI Official Synonym Symbols
HEL-S-79
NCBI Protein Information
claudin-8
UniProt Protein Name
Claudin-8
UniProt Gene Name
CLDN8
UniProt Entry Name
CLD8_HUMAN
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Product Notes
The Human CLDN8 cldn8 (Catalog #AAA331969) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA331969 ELISA Kit recognizes Human CLDN8. It is sometimes possible for the material contained within the vial of "Claudin-8 (CLDN8), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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