Human Collagenase I ELISA Kit

Human Collagenase I ELISA Kit

Cat. Num. AAA12542

• Reactivity: Human
• Synonyms: Collagenase I; N/A; Human Collagenase I ELISA Kit; Collagenase I elisa kit

• Reactivity: Human
• Specificity: No cross-reaction with other factors.
• Sequence Length: 900
• Assay Type: Sandwich
• Detection Range: 100 ng/mL-1.56 ng/mL
• Sensitivity: Up to 0.5 ng/mL

• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for Collagenase I elisa kit

Principle of the Assay: As mentioned above, this kit utilizes the Double Antibody Sandwich ELISA technique. The pre-coated antibody is an anti-Human Collagenase I monoclonal antibody, while the detection antibody is a biotinylated polyclonal antibody. Samples and biotinylated antibodies are added into ELISA plate wells and washed out with PBS or TBS after their respective additions to the wells. Then Avidin-peroxidase conjugates are added to the wells in after. TMB substrate is used for coloration after the enzyme conjugate has already been thoroughly washed out of the wells by PBS or TBS. TMB reacts to form a blue product from the peroxidase activity, and finally turns to yellow after addition of the stop solution (Color Reagent C). The color intensity and quantity of target analyte in the sample are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 32472801
• NCBI Accession #: NP_865795.1
• NCBI GenBank Nucleotide #: NC_005027.1
• NCBI Official Full Name: collagenase

Frequently Asked Questions

What does a human collagenase ELISA kit measure?

This ELISA kit quantitatively measures human collagenase (matrix metalloproteinases) protein levels in biological samples. Collagenase enzymes specifically degrade collagen and other extracellular matrix proteins, making them key biomarkers for tissue remodeling and degradation processes in tissues and body fluids.

Which collagenase enzymes can be detected with this ELISA kit?

The kit detects human collagenase Type I (MMP-1) and/or Type II enzymes, which are the primary collagenases responsible for cleaving native collagen in the extracellular matrix. These enzymes are ubiquitously produced by fibroblasts, macrophages, and other cell types during tissue remodeling and inflammation.

Is collagenase a marker for tissue remodeling or inflammation?

Yes, collagenase serves as both a tissue remodeling and an inflammation marker. Elevated collagenase levels indicate active extracellular matrix degradation occurring during normal wound healing and pathological inflammation, fibrosis, and tissue turnover in various disease states.

Can this kit be used to study extracellular matrix degradation?

Absolutely, Collagenase is the primary enzyme responsible for native collagen breakdown in ECM degradation. This kit directly measures collagenase activity levels, making it ideal for studying ECM remodeling dynamics in tissue engineering, wound healing, and disease models involving matrix degradation.

Is collagenase ELISA commonly used in fibrosis or cancer research?

Yes, collagenase measurement is extensively used in fibrosis research to monitor excessive collagen deposition and in cancer research to assess tumor microenvironment remodeling and metastatic potential, as elevated collagenase correlates with increased invasiveness and epithelial-mesenchymal transition.

What human sample types are typically tested for collagenase levels?

Standard sample types include serum, plasma, tissue homogenates, urine, and cell culture supernatants. For clinical diagnostics, 24-hour urine collection and plasma samples are most commonly used to assess systemic collagenase levels during disease monitoring.