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product-image-AAA187707_SC15.jpg Standard Curve (Sample)

Human Complement Factor H Related Protein 3 (CFHR3) ELISA Kit | CFHR3 elisa kit

Human Complement Factor H Related Protein 3 (CFHR3) ELISA Kit

Gene Names
CFHR3; FHR3; HLF4; CFHL3; FHR-3; DOWN16
Reactivity
Human
Synonyms
Complement Factor H Related Protein 3 (CFHR3); N/A; Human Complement Factor H Related Protein 3 (CFHR3) ELISA Kit; CFHL3; DOWN16; FHR-3; FHR3; HLF4; H factor-like protein 3; CFHR3 elisa kit
Ordering
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of CFHR3. No significant cross-reactivity or interference between CFHR3 and analogues was observed.
Sequence Length
269
Assay Type
Quantitative Sandwich
Samples
Human serum, plasma and other biological fluids
Detection Range
0.156-10ng/mL
Sensitivity
< 0.074ng/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level CFHR3 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level CFHR3 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Standard Curve (Sample)

product-image-AAA187707_SC15.jpg Standard Curve (Sample)
Related Product Information for CFHR3 elisa kit
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to CFHR3. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to CFHR3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CFHR3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of CFHR3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of CFHR3 in human serum, plasma and other biological fluids.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
30,720 Da
NCBI Official Full Name
complement factor H-related protein 3 isoform 1
NCBI Official Synonym Full Names
complement factor H related 3
NCBI Official Symbol
CFHR3
NCBI Official Synonym Symbols
FHR3; HLF4; CFHL3; FHR-3; DOWN16
NCBI Protein Information
complement factor H-related protein 3
UniProt Protein Name
Complement factor H-related protein 3
UniProt Gene Name
CFHR3
UniProt Synonym Gene Names
CFHL3; FHR3; FHR-3

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Product Notes

The Human CFHR3 cfhr3 (Catalog #AAA187707) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA187707 ELISA Kit recognizes Human CFHR3. It is sometimes possible for the material contained within the vial of "Complement Factor H Related Protein 3 (CFHR3), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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