Standard Curve (Sample)
Human Complement fragment 4b, C4b ELISA Kit | C4b elisa kit
Human Complement fragment 4b, C4b ELISA Kit
Gene Names
C4B; CH; C4F; CO4; C4B1; C4B2; C4B3; C4B5; C4BD; C4B12; C4B_2; CPAMD3
Reactivity
Human
Synonyms
Complement fragment 4b, C4b; N/A; Human Complement fragment 4b, C4b ELISA Kit; Human Complement fragment 4b; C4b ELISA Kit; Complement fragment 4b; C4b; C4b elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of human C4b. No significant cross-reactivity or interference between human C4b and analogues was observed.
Sequence Length
1744
Samples
Serum, plasma
Assay Type
Quantitative Sandwich
Detection Range
15.6 ng/ml-1000 ng/ml
Sensitivity
<3.9 ng/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for C4b elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for C4b has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any C4b present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for C4b is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of C4b bound in the initial step. The color development is stopped and the intensity of the color is measured.
References
Peptide Patterns as Discriminating Biomarkers in Plasma of Patients With Familial Adenomatous Polyposis. Lisa Agatea, Sara Crotti, Eugenio Ragazzi, Chiara Bedin, Emanuele Urso, Isabella Mammi, Pietro Traldi, Salvatore Pucciarelli, Donato Nitti, Marco Agostini (2015)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
192,751 Da
NCBI Official Full Name
complement C4-B preproprotein
NCBI Official Synonym Full Names
complement component 4B (Chido blood group)
NCBI Official Symbol
C4B
NCBI Official Synonym Symbols
CH; C4F; CO4; C4B1; C4B2; C4B3; C4B5; C4BD; C4B12; C4B_2; CPAMD3
NCBI Protein Information
complement C4-B; Chido form of C4; complement C4B1a; basic complement C4; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 3
UniProt Protein Name
Complement C4-B
UniProt Gene Name
C4B
UniProt Synonym Gene Names
CO4; CPAMD3
C4B_2
C4B_2
UniProt Entry Name
CO4B_HUMAN
Similar Products
Product Notes
The Human C4b c4b (Catalog #AAA15006) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA15006 ELISA Kit recognizes Human C4b. It is sometimes possible for the material contained within the vial of "Complement fragment 4b, C4b, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.