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product-image-AAA188269_SC15.jpg Standard Curve (Sample)

Human Coproporphyrinogen Oxidase (CPOX) ELISA Kit | CPOX elisa kit

Human Coproporphyrinogen Oxidase (CPOX) ELISA Kit

Gene Names
CPOX; COX; CPO; CPX; HCP
Reactivity
Human
Synonyms
Coproporphyrinogen Oxidase (CPOX); N/A; Human Coproporphyrinogen Oxidase (CPOX) ELISA Kit; HCP; CPO; CPX; Coproporphyrinogenase; Coproporphyria; Harderoporphyria; Oxygen-dependent coproporphyrinogen-III oxidase; mitochondrial; CPOX elisa kit
Ordering
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of Coproporphyrinogen Oxidase (CPOX).
No significant cross-reactivity or interference between Coproporphyrinogen Oxidase (CPOX) and analogues was observed.
Sequence Length
454
Assay Type
Double-antibody Sandwich
Samples
Tissue Homogenates, Cell Lysates and Other Biological Fluids
Detection Range
0.156-10ng/mL
Sensitivity
The minimum detectable dose of this kit is typically less than 0.055ng/mL
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Coproporphyrinogen Oxidase (CPOX) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Coproporphyrinogen Oxidase (CPOX) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Standard Curve (Sample)

product-image-AAA188269_SC15.jpg Standard Curve (Sample)
Related Product Information for CPOX elisa kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Coproporphyrinogen Oxidase (CPOX). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Coproporphyrinogen Oxidase (CPOX). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Coproporphyrinogen Oxidase (CPOX), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Coproporphyrinogen Oxidase (CPOX) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Oxygen-dependent coproporphyrinogen-III oxidase, mitochondrial
NCBI Official Synonym Full Names
coproporphyrinogen oxidase
NCBI Official Symbol
CPOX
NCBI Official Synonym Symbols
COX; CPO; CPX; HCP
NCBI Protein Information
oxygen-dependent coproporphyrinogen-III oxidase, mitochondrial
UniProt Protein Name
Oxygen-dependent coproporphyrinogen-III oxidase, mitochondrial
UniProt Gene Name
CPOX
UniProt Synonym Gene Names
CPO; CPX; COX; Coprogen oxidase; Coproporphyrinogenase
UniProt Entry Name
HEM6_HUMAN

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Product Notes

The Human CPOX cpox (Catalog #AAA188269) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA188269 ELISA Kit recognizes Human CPOX. It is sometimes possible for the material contained within the vial of "Coproporphyrinogen Oxidase (CPOX), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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