Human cross linked N-telopeptide of type I collagen, NTX ELISA Kit | NTX elisa kit
Human cross linked N-telopeptide of type I collagen, NTX ELISA Kit
Reactivity
Human
Synonyms
cross linked N-telopeptide of type I collagen, NTX; N/A; Human cross linked N-telopeptide of type I collagen, NTX ELISA Kit; cross linked N-telopeptide of type I collagen, NTX ELISA Kit (Human); NTX elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of NTX. No significant cross-reactivity or interference between NTX and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between NTX and all the analogues, therefore, cross reaction may still exist in some cases.
Assay Type
Quantitative Competitive
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Sensitivity
1.0 ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C
Standard Curve (Sample)
Related Product Information for NTX elisa kit
Principle of the Assay: NTX ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-NTX antibody and an NTX-HRP conjugate. The assay sample and buffer are incubated together with NTX-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the NTX concentration since NTX from samples and NTX-HRP conjugate compete for the anti-NTX antibody binding site. Since the number of sites is limited, as more sites are occupied by NTX from the sample, fewer sites are left to bind NTX-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NTX concentration in each sample is interpolated from this standard curve.
Intended Uses: This NTX ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human NTX. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Intended Uses: This NTX ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human NTX. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Product Categories/Family for NTX elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
NTX
