Human CXCL14 ELISA Kit | CXCL14 elisa kit

Human CXCL14 PicoKine ELISA Kit

Cat. Num. AAA45887

• Gene Names: CXCL14; KEC; KS1; BMAC; BRAK; NJAC; MIP2G; MIP-2g; SCYB14
• Reactivity: Human
• Synonyms: CXCL14; N/A; Human CXCL14 PicoKine ELISA Kit; C-X-C motif chemokine 14; chemokine (C-X-C motif) ligand 14; 1110031L23Rik; 1200006I23Rik; AI414372; BMAC; bolekine; BRAK; Chaemokine, CXC motif, ligand 14; Chemokine (C-X-C motif) ligand 14; Chemokine BRAK; CXC chemokine in breast and kidney; Cxcl14; CXL14_HUMAN; JSC; Kec; Kidney-expressed chemokine CXC; KS1; MGC10687; MGC124510; MGC90667; MIP 2 gamma; MIP-2G; MIP2G; MIP2gamma; NJAC; PRO273; PSEC0212; Scyb14; Small Inducible Cytokine B14; Small inducible cytokine subfamily B (Cys-X-Cys) member 14 (BRAK); Small Inducible Cytokine subfamily B, member 14; Small-inducible cytokine B14; UNQ240; CXCL14 elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant human CXCL14
• Sequence Length: 111
• Samples: Cell culture supernates, serum and plasma (heparin, EDTA)
• Assay Type: Sandwich
• Detection Range: 62.5pg/ml-4000pg/ml
• Sensitivity: < 10pg/ml

• Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Standard Curve (Sample)

(Human CXCL14 PicoKine ELISA Kit standard curve)

Related Product Information for CXCL14 elisa kit

Principle of the assay: human CXCL14 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CXCL14 has been precoated onto 96-well plates. Standards(E.coli, S35-E111) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CXCL14 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CXCL14 amount of sample captured in plate.
Background: Chemokine(C-X-C motif) ligand 14(CXCL14) is a small cytokine belonging to the CXC chemokine family that is also known as BRAK(for breast and kidney-expressed chemokine). This gene is mapped to 5q31.1. CXCL14 is constitutively expressed at high levels in many normal tissues, it has been found that fibroblasts are the primary source of CXCL14 and that CXCL14 is involved in the homeostasis of monocyte-derived macrophages rather than in inflammation. It is also a potent chemoattractant and activator of dendritic cells, is implicated in homing of these cells, and can stimulate the migration of activated NK cells. CXCL14 also inhibits angiogenesis, possibly as a result of its ability to block endothelial cell chemotaxis. In addition to it, CXCL14 is a potent chemokine for immature DCs, with a chemoattractive activity comparable to bacterial fMLP and MIP1A.

NCBI and Uniprot Product Information

• NCBI GI #: 292495003
• NCBI GeneID: 9547
• UniProt Accession #: O95715; Q6UW97; Q86U69; Q9BTR1; Q9NS21; B3KQU8
• Molecular Weight: 13,078 Da
• NCBI Official Full Name: C-X-C motif chemokine 14
• NCBI Official Synonym Full Names: chemokine (C-X-C motif) ligand 14
• NCBI Official Symbol: CXCL14
• NCBI Official Synonym Symbols: KEC; KS1; BMAC; BRAK; NJAC; MIP2G; MIP-2g; SCYB14
• NCBI Protein Information: C-X-C motif chemokine 14; bolekine; MIP-2 gamma; chemokine BRAK; breast and kidney; tumor-suppressing chemokine; small-inducible cytokine B14; CXC chemokine in breast and kidney; small inducible cytokine subfamily B (Cys-X-Cys), member 14 (BRAK)
• UniProt Protein Name: C-X-C motif chemokine 14
• UniProt Gene Name: CXCL14
• UniProt Synonym Gene Names: MIP2G; NJAC; SCYB14
• UniProt Entry Name: CXL14_HUMAN