Standard Curve (Sample)
Human Cystathionine gamma-Lyase (CTH) ELISA Kit | CTH elisa kit
Human Cystathionine gamma-Lyase (CTH) ELISA Kit
Reactivity
Human
Synonyms
Cystathionine gamma-Lyase (CTH); N/A; Human Cystathionine gamma-Lyase (CTH) ELISA Kit; CTH; MGC9471; cystathionase; cysteine desulfhydrase; gamma-cystathionase; homoserine deaminase; homoserine dehydratase; CTH elisa kit
Reactivity
Human
Specificity
Human Cystathionine gamma-lyase (CTH) ELISA Kit has high sensitivity and excellent specificity for detection of Human CTH. No significant cross-reactivity or interference between Human CTH and analogues was observed.
Sequence Length
405
Assay Type
Sandwich
Samples
Human serum, cell culture supernates and other biological fluids
Detection Range
5 umol/L - 80 umol/L
Sensitivity
< 0.5umol/L
Intra-assay Precision
Four samples of known concentration were tested twenty times on one plate to assess intra-assay precision. The CV (%)?9%.
Inter-assay Precision
Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components. The CV (%)?11%.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for CTH elisa kit
Background: CSE is an enzyme which breaks down cystathionine into cysteine and alpha-ketobutyrate. Pyridoxal phosphate is a prosthetic group of this enzyme.The bioavailability of cysteine is rate-limiting for the hepatic synthesis of glutathione. Cysteine is provided through either the diet or the transsulfuration pathway, in which cystathionine derived from methionine is transformed into cysteine by cystathionine gamma-lyase.Mutant mice lacking CTH displayed pronounced hypertension and diminished endothelium-dependent vasorelaxation. Cystathionine was physiologically activated by calcium and calmodulin, which is a mechanism for H2S formation in response to vascular activation.
Principle of the Assay: Human Cystathionine gamma-lyase (CTH) ELISA Kit employs a two-site sandwich ELISA to quantitate CTH in samples. An antibody specific for CTH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CTH present is bound by the immobilized antibody. After removing any unbound substances, HRP-Conjugated Human CTH detection antibody is added to the wells. Following a wash to remove any unbound HRP reagent, a Chromogen solution is added to the wells and color develops in proportion to the amount of CTH bound in the initial step. The color development is stopped and the intensity of the color is measured.
Principle of the Assay: Human Cystathionine gamma-lyase (CTH) ELISA Kit employs a two-site sandwich ELISA to quantitate CTH in samples. An antibody specific for CTH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CTH present is bound by the immobilized antibody. After removing any unbound substances, HRP-Conjugated Human CTH detection antibody is added to the wells. Following a wash to remove any unbound HRP reagent, a Chromogen solution is added to the wells and color develops in proportion to the amount of CTH bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Cystathionine gamma-lyase
NCBI Official Synonym Full Names
cystathionine gamma-lyase
NCBI Official Symbol
CTH
NCBI Protein Information
cystathionine gamma-lyase
UniProt Protein Name
Cystathionine gamma-lyase
UniProt Gene Name
CTH
UniProt Entry Name
CGL_HUMAN
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Product Notes
The Human CTH cth (Catalog #AAA331967) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA331967 ELISA Kit recognizes Human CTH. It is sometimes possible for the material contained within the vial of "Cystathionine gamma-Lyase (CTH), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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