Human Cysteine rich C terminal protein 1 (CRCT1) ELISA Kit | CRCT1 elisa kit

Human Cysteine rich C terminal protein 1 (CRCT1) ELISA Kit

Cat. Num. AAA245489

• Gene Names: CRCT1; NICE-1; C1orf42
• Reactivity: Human
• Synonyms: Cysteine rich C terminal protein 1 (CRCT1); N/A; Human Cysteine rich C terminal protein 1 (CRCT1) ELISA Kit; CRCT1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of CRCT1. No significant cross-reactivity or interference between CRCT1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CRCT1 and all the analogues, therefore, cross reaction may still exist in some cases.
• Assay Type: Quantitative Competitive
• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Sensitivity: 1.0 ng/mL

• Preparation and Storage: Store all reagents at 2-8 degree C.

Standard Curve (Sample)

Related Product Information for CRCT1 elisa kit

Principle of the Assay: CRCT1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CRCT1 antibody and an CRCT1-HRP conjugate. The assay sample and buffer are incubated together with CRCT1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CRCT1 concentration since CRCT1 from samples and CRCT1-HRP conjugate compete for the anti-CRCT1 antibody binding site. Since the number of sites is limited, as more sites are occupied by CRCT1 from the sample, fewer sites are left to bind CRCT1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CRCT1 concentration in each sample is interpolated from this standard curve.

Intended Uses: This CRCT1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CRCT1. This ELISA kit for research use only, not for therapeutic or test applications!

Product Categories/Family for CRCT1 elisa kit

Cell Biology

NCBI and Uniprot Product Information

• NCBI GI #: 9506923
• NCBI GeneID: 54544
• NCBI Accession #: NP_061933.1
• NCBI GenBank Nucleotide #: NM_019060.2
• Molecular Weight: 9,736 Da
• NCBI Official Full Name: cysteine-rich C-terminal protein 1
• NCBI Official Synonym Full Names: cysteine-rich C-terminal 1
• NCBI Official Symbol: CRCT1
• NCBI Official Synonym Symbols: NICE-1; C1orf42
• NCBI Protein Information: cysteine-rich C-terminal protein 1; protein NICE-1
• UniProt Protein Name: Cysteine-rich C-terminal protein 1
• UniProt Gene Name: CRCT1
• UniProt Synonym Gene Names: C1orf42; NICE1
• UniProt Entry Name: CRCT1_HUMAN