Standard Curve (Sample)
(Fig.1. Human Cytochrome P450 2D6 (CYP2D6) Standard Curve.)
Human Cytochrome P450 2D6 (CYP2D6) ELISA Kit | CYP2D6 elisa kit
Human Cytochrome P450 2D6 (CYP2D6) ELISA Kit
Gene Names
CYP2D6; CPD6; CYP2D; CYP2DL1; CYPIID6; P450C2D; P450DB1; CYP2D7AP; CYP2D7BP; CYP2D7P2; CYP2D8P2; P450-DB1
Reactivity
Human
Synonyms
Cytochrome P450 2D6 (CYP2D6); N/A; Human Cytochrome P450 2D6 (CYP2D6) ELISA Kit; CYP2D6; RP4-669P10.2; CPD6; CYP2D; CYP2DL1; MGC120389; MGC120390; P450-DB1; P450C2D; P450DB1; cytochrome P450 2D6; cytochrome P450; subfamilyD (debrisoquine; sparteine; etc; -metabolizing); polyp; CYP2D6 elisa kit
Reactivity
Human
Specificity
Human Cytochrome P450 2D6 (CYP2D6) ELISA Kit has high sensitivity and excellent specificity for detection of Human CYP2D6. No significant cross-reactivity or interference between Human CYP2D6 and analogues was observed.
Sequence Length
497
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human Cytochrome P450 2D6 (CYP2D6) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human Cytochrome P450 2D6 (CYP2D6) Standard Curve.)
Related Product Information for CYP2D6 elisa kit
Description: This Human Cytochrome P450 2D6 (CYP2D6) ELISA Kit employs a two-site sandwich ELISA to quantitate CYP2D6 in samples. An antibody specific for CYP2D6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CYP2D6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CYP2D6 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CYP2D6 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Cytochrome P450 2D6 (CYP2D6), a member of the cytochrome P450 mixed-function oxidase system, is one of the most important enzymes involved in the metabolism of xenobiotics in the body. Whilst CYP2D6 is involved in the oxidation of a wide range of substrates of all the CYPs, there is considerable variability in its expression in the liver. The gene is located near two cytochrome P450 pseudogenes on chromosome 22q13.1. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. CYP2D6 activity was tested in "healthy infants receiving an oral dose (0.3 mg/kg) of dextromethorphan (DM) at 0.5, 1, 2, 4, 6, and 12 months of age. DM and its major metabolites were measured in urine. CYP2D6 genotype was determined by polymerase chain reaction-restriction fragment length polymorphism.
Background: Cytochrome P450 2D6 (CYP2D6), a member of the cytochrome P450 mixed-function oxidase system, is one of the most important enzymes involved in the metabolism of xenobiotics in the body. Whilst CYP2D6 is involved in the oxidation of a wide range of substrates of all the CYPs, there is considerable variability in its expression in the liver. The gene is located near two cytochrome P450 pseudogenes on chromosome 22q13.1. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. CYP2D6 activity was tested in "healthy infants receiving an oral dose (0.3 mg/kg) of dextromethorphan (DM) at 0.5, 1, 2, 4, 6, and 12 months of age. DM and its major metabolites were measured in urine. CYP2D6 genotype was determined by polymerase chain reaction-restriction fragment length polymorphism.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
cytochrome P450 2D6 isoform 1
NCBI Official Synonym Full Names
cytochrome P450 family 2 subfamily D member 6
NCBI Official Symbol
CYP2D6
NCBI Official Synonym Symbols
CPD6; CYP2D; CYP2DL1; CYPIID6; P450C2D; P450DB1; CYP2D7AP; CYP2D7BP; CYP2D7P2; CYP2D8P2; P450-DB1
NCBI Protein Information
cytochrome P450 2D6
UniProt Protein Name
Cytochrome P450 2D6
UniProt Gene Name
CYP2D6
UniProt Synonym Gene Names
CYP2DL1
UniProt Entry Name
CP2D6_HUMAN
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Product Notes
The Human CYP2D6 cyp2d6 (Catalog #AAA331977) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA331977 ELISA Kit recognizes Human CYP2D6. It is sometimes possible for the material contained within the vial of "Cytochrome P450 2D6 (CYP2D6), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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