Application Data
(This assay recognizes both recombinant and natural human CD209. The factors listed above were prepared at 50ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.A sample containing 50ng/mL of recombinant human CLEC4M reads as 0.7ng/mL(1.4% cross-reactivity).Preparations of ICAM2, ICAM3, CLU, ICAM5 and LSP1 at 20ng/mL in a mid-range recombinant human CD209 control were assayed for interference. No significant interference was observed.)
Human DC-SIGN/CD209 ELISA Kit | DC-SIGN/CD209 elisa kit
Human DC-SIGN/CD209 ELISA Kit
Reactivity
Human
Synonyms
DC-SIGN/CD209; N/A; Human DC-SIGN/CD209 ELISA Kit; DC-SIGN ELISA Kit, Human; CD209 molecule; CD209 ELISA Kit, Human; CDSIGN ELISA Kit, Human; CLEC4L ELISA Kit, Human; DC-SIGN1 ELISA Kit, Human; MGC129965 ELISA Kit, Human; DC-SIGN/CD209 elisa kit
Reactivity
Human
Specificity
For the quantitative determination of Human DC-SIGN/CD209 concentration in serum.
Assay Type
Solid Phase Sandwich ELISA (quantitative)
Sample Types
Serum
Detection Range
31.25-2000pg/mL
Sensitivity
11.8pg/mL
Conjugation
HRP
Interference
Preparations of ICAM2, ICAM3, CLU, ICAM5 and LSP1 at 20ng/mL in a mid-range recombinant human CD209 control were assayed for interference. No significant interference was observed.
Materials Provided
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
Application Data
(This assay recognizes both recombinant and natural human CD209. The factors listed above were prepared at 50ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.A sample containing 50ng/mL of recombinant human CLEC4M reads as 0.7ng/mL(1.4% cross-reactivity).Preparations of ICAM2, ICAM3, CLU, ICAM5 and LSP1 at 20ng/mL in a mid-range recombinant human CD209 control were assayed for interference. No significant interference was observed.)
Application Data
(This assay recognizes both recombinant and natural human CD209. The factors listed above were prepared at 50ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.A sample containing 50ng/mL of recombinant human CLEC4M reads as 0.7ng/mL(1.4% cross-reactivity).Preparations of ICAM2, ICAM3, CLU, ICAM5 and LSP1 at 20ng/mL in a mid-range recombinant human CD209 control were assayed for interference. No significant interference was observed.)
Standard Curve (Sample)
(This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.)
Standard Curve (Sample)
(This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.)
Related Product Information for DC-SIGN/CD209 elisa kit
This DC-SIGN ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human DC-SIGN protein in Serum. It contains recombinant Human DC-SIGN, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
Background: Dendritic cell (DC)-specific intercellular adhesion molecule 3 (ICAM-3) grabbing nonintegrin (DC-SIGN), also known as CD209, is a type II transmembrane protein on DCs with a C-type lectin extracellular domain, is capable of binding ICAM-3 on resting T cells in the secondary lymphoid organs, providing the initial contact between these cells during the establishment of cell-mediated immunity. It is not only a pattern recognition receptor but implicated in immunoregulation of DCs. It has an important role in mediating DC adhesion, migration, inflammation, activating primary T cell, triggering immune response and participating in immune escape of pathogens and tumors. DC-SIGN also mediates the capture and internalization of viral, bacterial, and fungal pathogens by dendritic cells, such as HIV-1, Ebola virus, cytomegalovirus, Dengue virus, and hepatitis C virus. DC-SIGN is unique in that it regulates adhesion processes, such as DC trafficking and T-cell synapse formation, as well as antigen capture. Moreover, even though several C-type lectins have been shown to bind HIV-1, DC-SIGN does not only capture HIV-1 but also protects it in early endosomes allowing HIV-1 transport by DC to lymphoid tissues, where it enhances trans infection of T cells.
Background: Dendritic cell (DC)-specific intercellular adhesion molecule 3 (ICAM-3) grabbing nonintegrin (DC-SIGN), also known as CD209, is a type II transmembrane protein on DCs with a C-type lectin extracellular domain, is capable of binding ICAM-3 on resting T cells in the secondary lymphoid organs, providing the initial contact between these cells during the establishment of cell-mediated immunity. It is not only a pattern recognition receptor but implicated in immunoregulation of DCs. It has an important role in mediating DC adhesion, migration, inflammation, activating primary T cell, triggering immune response and participating in immune escape of pathogens and tumors. DC-SIGN also mediates the capture and internalization of viral, bacterial, and fungal pathogens by dendritic cells, such as HIV-1, Ebola virus, cytomegalovirus, Dengue virus, and hepatitis C virus. DC-SIGN is unique in that it regulates adhesion processes, such as DC trafficking and T-cell synapse formation, as well as antigen capture. Moreover, even though several C-type lectins have been shown to bind HIV-1, DC-SIGN does not only capture HIV-1 but also protects it in early endosomes allowing HIV-1 transport by DC to lymphoid tissues, where it enhances trans infection of T cells.
References
Geijtenbeek TB, et al. (2002) DC-SIGN, a C-type lectin on dendritic cells that unveils many aspects of dendritic cell biology. J Leukoc Biol. 71(6): 921-31.
Masso M. (2003) DC-SIGN points the way to a novel mechanism for HIV-1 transmission. MedGenMed. 5(2): 2.
Zhou T, et al. (2006) DC-SIGN and immunoregulation. Cell Mol Immunol. 3(4): 279-83.
Masso M. (2003) DC-SIGN points the way to a novel mechanism for HIV-1 transmission. MedGenMed. 5(2): 2.
Zhou T, et al. (2006) DC-SIGN and immunoregulation. Cell Mol Immunol. 3(4): 279-83.
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Product Notes
The Human DC-SIGN/CD209 (Catalog #AAA258344) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA258344 ELISA Kit recognizes Human DC-SIGN/CD209. It is sometimes possible for the material contained within the vial of "DC-SIGN/CD209, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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