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product-image-AAA46186_SC15.png Standard Curve (Sample) (Human DCR3/TNFRSF6B PicoKine ELISA Kit standard curve)

Human DCR3/TNFRSF6B PicoKine ELISA Kit | TNFRSF6B elisa kit

Human DCR3/TNFRSF6B PicoKine ELISA Kit

Gene Names
TNFRSF6B; M68; TR6; DCR3; M68E; DJ583P15.1.1
Reactivity
Human
Synonyms
DCR3/TNFRSF6B PicoKine; N/A; Human DCR3/TNFRSF6B PicoKine ELISA Kit; Tumor necrosis factor receptor superfamily member 6B; DcR3; Decoy Receptor 3; Decoy receptor for Fas ligand; DJ583P15.1.1; M68; M68E; OTTHUMP00000031583; TNF6B_HUMAN; TNFRSF6B; TR6; Tumor necrosis factor receptor superfamily member 6b decoy; UNQ186/PRO212 ; tumor necrosis factor receptor superfamily, member 6b, decoy; TNFRSF6B elisa kit
Ordering
Reactivity
Human
Specificity
Natural and recombinant human OCR3
Sequence Length
300
Samples
Cell culture supernates, cell Iysates, tissue homogenates, serum and plasma (heparin, EDTA)
Assay Type
Quantitative Sandwich
Detection Range
156pg!ml-10000pg/ml
Sensitivity
< 10pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay rrecision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Standard Curve (Sample)

(Human DCR3/TNFRSF6B PicoKine ELISA Kit standard curve)

product-image-AAA46186_SC15.png Standard Curve (Sample) (Human DCR3/TNFRSF6B PicoKine ELISA Kit standard curve)
Related Product Information for TNFRSF6B elisa kit
Principle of the Assay: The human DCR3 ELISA Kit was based on standard sandwich enzyme-lirked immune-sorbent assay technology. A monoclonal antibody from mouse specific for DCR3 has been precoate-J onto 96-well plates. Standards (Expression system for standard: sf21. Immunogen sequence: V24 - H300) and test samples are added to the wells, a biotinylated detection polyc!onal antibody from goat specific for DCR3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human DCR3 amount of sample captured in plate.

Background/Introduction: Decoy receptor 3 (Dcr3), also known as tumor necrosis factor receptor superfamily member 6B (TNFRSF6B), TR6 and M68, is a soluble protein of the tumor necrosis factor receptor superfamily which inhibits Fas ligand-inducedapoptosis. This gene belongs to the tumor necrosis factor receptor superfamily. It acts as a decoy receptor that competes with death receptors for ligand binding. The encoded protein is postulated to play a regulatory role in suppressing FasL- and LIGHT-mediated cell death and T cell activation as well as to induce angiogenesis via neutralization of TL 1A. Overexpression of this gene has be':!n noted in various tumors e.g. gastrointestinal tract tumors, and it is located in a gene-rich cluster on chromosome 20, with other potentially tumor-related genes.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
32,680 Da
NCBI Official Full Name
tumor necrosis factor receptor superfamily member 6B
NCBI Official Synonym Full Names
tumor necrosis factor receptor superfamily member 6b
NCBI Official Symbol
TNFRSF6B
NCBI Official Synonym Symbols
M68; TR6; DCR3; M68E; DJ583P15.1.1
NCBI Protein Information
tumor necrosis factor receptor superfamily member 6B
UniProt Protein Name
Tumor necrosis factor receptor superfamily member 6B
UniProt Gene Name
TNFRSF6B
UniProt Synonym Gene Names
DCR3; TR6; DcR3
UniProt Entry Name
TNF6B_HUMAN

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Product Notes

The Human TNFRSF6B tnfrsf6b (Catalog #AAA46186) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA46186 ELISA Kit recognizes Human TNFRSF6B. It is sometimes possible for the material contained within the vial of "DCR3/TNFRSF6B PicoKine, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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