Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of human DEK. No significant cross-reactivity or interference between human DEK and analogues was observed.
Sequence Length
341
Assay Type
Quantitative Sandwich
Samples
Serum, plasma, tissue homogenates, cell lysates
Detection Range
23.44 pg/ml-1500 pg/ml
Sensitivity
< 5.86 pg/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Related Product Information for DEK elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for DEK has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DEK present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DEK is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DEK bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
42,674 Da
NCBI Official Full Name
protein DEK isoform 2
NCBI Official Synonym Full Names
DEK proto-oncogene
NCBI Official Symbol
DEK
NCBI Official Synonym Symbols
D6S231E
NCBI Protein Information
protein DEK; DEK oncogene (DNA binding)
UniProt Protein Name
Protein DEK
UniProt Gene Name
DEK
UniProt Entry Name
DEK_HUMAN