Standard Curve (Sample)
(Fig.1. Human Delta-aminolevulinic acid dehydratase (ALAD) Standard Curve.)
Human Delta-Aminolevulinic Acid Dehydratase (ALAD) ELISA Kit | ALAD elisa kit
Human Delta-Aminolevulinic Acid Dehydratase (ALAD) ELISA Kit
Gene Names
ALAD; PBGS; ALADH
Reactivity
Human
Synonyms
Delta-Aminolevulinic Acid Dehydratase (ALAD); N/A; Human Delta-Aminolevulinic Acid Dehydratase (ALAD) ELISA Kit; ALAD; RP11-10I9.1; ALADH; MGC5057; PBGS; aminolevulinate dehydratase; delta-aminolevulinic acid dehydratase; porphobilinogen synthase; ALAD elisa kit
Reactivity
Human
Specificity
Human Delta-aminolevulinic acid dehydratase (ALAD) ELISA Kit has high sensitivity and excellent specificity for detection of Human ALAD. No significant cross-reactivity or interference between Human ALAD and analogues was observed.
Sequence Length
339
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human Delta-aminolevulinic acid dehydratase (ALAD) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human Delta-aminolevulinic acid dehydratase (ALAD) Standard Curve.)
Related Product Information for ALAD elisa kit
Description: This Human Delta-aminolevulinic acid dehydratase (ALAD) ELISA Kit employs a two-site sandwich ELISA to quantitate ALAD in samples. An antibody specific for ALAD has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ALAD present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ALAD is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ALAD bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: The ALAD enzyme is composed of 8 identical subunits and catalyzes the condensation of 2 molecules of delta-aminolevulinate to form porphobilinogen (a precursor of heme, cytochromes and other hemoproteins). ALAD catalyzes the second step in the porphyrin and heme biosynthetic pathway; zinc is essential for enzymatic activity. ALAD enzymatic activity is inhibited by lead and a defect in the ALAD structural gene can cause increased sensitivity to lead poisoning and acute hepatic porphyria. Alternatively spliced transcript variants encoding different isoforms have been identified.
Background: The ALAD enzyme is composed of 8 identical subunits and catalyzes the condensation of 2 molecules of delta-aminolevulinate to form porphobilinogen (a precursor of heme, cytochromes and other hemoproteins). ALAD catalyzes the second step in the porphyrin and heme biosynthetic pathway; zinc is essential for enzymatic activity. ALAD enzymatic activity is inhibited by lead and a defect in the ALAD structural gene can cause increased sensitivity to lead poisoning and acute hepatic porphyria. Alternatively spliced transcript variants encoding different isoforms have been identified.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
aminolevulinate, delta-, dehydratase, isoform CRA_a
NCBI Official Synonym Full Names
aminolevulinate dehydratase
NCBI Official Symbol
ALAD
NCBI Official Synonym Symbols
PBGS; ALADH
NCBI Protein Information
delta-aminolevulinic acid dehydratase
UniProt Protein Name
Delta-aminolevulinic acid dehydratase
UniProt Gene Name
ALAD
UniProt Synonym Gene Names
ALADH
UniProt Entry Name
HEM2_HUMAN
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Product Notes
The Human ALAD alad (Catalog #AAA330929) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA330929 ELISA Kit recognizes Human ALAD. It is sometimes possible for the material contained within the vial of "Delta-Aminolevulinic Acid Dehydratase (ALAD), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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