Standard Curve (Sample)
(Fig.1. Human Protein disulfide-isomerase TMX3 (TMX3) Standard Curve.)
Human Disulfide-Isomerase TMX3 (TMX3) ELISA Kit | TMX3 elisa kit
Human Protein Disulfide-Isomerase TMX3 (TMX3) ELISA Kit
Gene Names
TMX3; PDIA13; TXNDC10
Reactivity
Human
Synonyms
Disulfide-Isomerase TMX3 (TMX3); N/A; Human Protein Disulfide-Isomerase TMX3 (TMX3) ELISA Kit; TMX3; FLJ20793; KIAA1830; PDIA13; TXNDC10; protein disulfide isomerase family A; member 13; thioredoxin domain containing 10; TMX3 elisa kit
Reactivity
Human
Specificity
Human Protein disulfide-isomerase TMX3 (TMX3) ELISA Kit has high sensitivity and excellent specificity for detection of Human TMX3. No significant cross-reactivity or interference between Human TMX3 and analogues was observed.
Sequence Length
454
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human Protein disulfide-isomerase TMX3 (TMX3) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human Protein disulfide-isomerase TMX3 (TMX3) Standard Curve.)
Related Product Information for TMX3 elisa kit
Description: This Human Protein disulfide-isomerase TMX3 (TMX3) ELISA Kit employs a two-site sandwich ELISA to quantitate TMX3 in samples. An antibody specific for TMX3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TMX3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TMX3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMX3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: The TMX3 gene encodes a protein of 454 amino acid residues that contains a predicted N-terminal signal sequence, a single domain with sequence similarity to thioredoxin and a CGHC active site sequence, a potential transmembrane domain, and a C-terminal KKKD tetrapeptide sequence that matches the classical KKXX-type consensus sequence for ER retrieval of type I transmembrane proteins. Endogenous TMX3 contains endoglycosidase H-sensitive glycans, localizes to the ER by Immunofluorescence microscopy, and is present in the membrane fraction after alkaline extraction of the ER luminal content. The TMX3 transcript is found in a variety of tissues and is not up-regulated by the unfolded protein response.
Background: The TMX3 gene encodes a protein of 454 amino acid residues that contains a predicted N-terminal signal sequence, a single domain with sequence similarity to thioredoxin and a CGHC active site sequence, a potential transmembrane domain, and a C-terminal KKKD tetrapeptide sequence that matches the classical KKXX-type consensus sequence for ER retrieval of type I transmembrane proteins. Endogenous TMX3 contains endoglycosidase H-sensitive glycans, localizes to the ER by Immunofluorescence microscopy, and is present in the membrane fraction after alkaline extraction of the ER luminal content. The TMX3 transcript is found in a variety of tissues and is not up-regulated by the unfolded protein response.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
protein disulfide-isomerase TMX3 isoform 1
NCBI Official Synonym Full Names
thioredoxin related transmembrane protein 3
NCBI Official Symbol
TMX3
NCBI Official Synonym Symbols
PDIA13; TXNDC10
NCBI Protein Information
protein disulfide-isomerase TMX3
UniProt Protein Name
Protein disulfide-isomerase TMX3
UniProt Gene Name
TMX3
UniProt Synonym Gene Names
KIAA1830; TXNDC10
UniProt Entry Name
TMX3_HUMAN
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Product Notes
The Human TMX3 tmx3 (Catalog #AAA330459) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA330459 ELISA Kit recognizes Human TMX3. It is sometimes possible for the material contained within the vial of "Disulfide-Isomerase TMX3 (TMX3), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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