Standard Curve (Sample)
(Fig.1. Human DNA repair protein RAD51 homolog 1 (RAD51) Standard Curve.)
Human DNA Repair Protein RAD51 Homolog 1 (RAD51) ELISA Kit | RAD51 elisa kit
Human DNA Repair Protein RAD51 Homolog 1 (RAD51) ELISA Kit
Gene Names
RAD51; RECA; BRCC5; FANCR; MRMV2; HRAD51; RAD51A; HsRad51; HsT16930
Reactivity
Human
Synonyms
DNA Repair Protein RAD51 Homolog 1 (RAD51); N/A; Human DNA Repair Protein RAD51 Homolog 1 (RAD51) ELISA Kit; RAD51; BRCC5; HRAD51; HsRad51; HsT16930; RAD51A; RECA; BRCA1/BRCA2-containing complex; subunit 5; DNA repair protein RAD51 homolog 1; RAD51 homolog protein; RecA; E. coli; homolog of; RecA-like protein; recombi; RAD51 elisa kit
Reactivity
Human
Specificity
Human DNA repair protein RAD51 homolog 1 (RAD51) ELISA Kit has high sensitivity and excellent specificity for detection of Human RAD51. No significant cross-reactivity or interference between Human RAD51 and analogues was observed.
Sequence Length
340
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human DNA repair protein RAD51 homolog 1 (RAD51) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human DNA repair protein RAD51 homolog 1 (RAD51) Standard Curve.)
Related Product Information for RAD51 elisa kit
Description: This Human DNA repair protein RAD51 homolog 1 (RAD51) ELISA Kit employs a two-site sandwich ELISA to quantitate RAD51 in samples. An antibody specific for RAD51 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any RAD51 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RAD51 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RAD51 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: RAD51 is a member of the RAD51 protein family. RAD51 family members are highly similar to bacterial RecA and Saccharomyces cerevisiae Rad51, and are known to be involved in the homologous recombination and repair of DNA. This protein can interact with the ssDNA-binding protein RPA and RAD52, and it is thought to play roles in homologous pairing and strand transfer of DNA. This protein is also found to interact with BRCA1 and BRCA2, which may be important for the cellular response to DNA damage. BRCA2 is shown to regulate both the intracellular localization and DNA-binding ability of this protein. Loss of these controls following BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis. Multiple transcript variants encoding different isoforms have been found for this gene.
Background: RAD51 is a member of the RAD51 protein family. RAD51 family members are highly similar to bacterial RecA and Saccharomyces cerevisiae Rad51, and are known to be involved in the homologous recombination and repair of DNA. This protein can interact with the ssDNA-binding protein RPA and RAD52, and it is thought to play roles in homologous pairing and strand transfer of DNA. This protein is also found to interact with BRCA1 and BRCA2, which may be important for the cellular response to DNA damage. BRCA2 is shown to regulate both the intracellular localization and DNA-binding ability of this protein. Loss of these controls following BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis. Multiple transcript variants encoding different isoforms have been found for this gene.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
DNA repair protein RAD51 homolog 1 isoform 2
NCBI Official Synonym Full Names
RAD51 recombinase
NCBI Official Symbol
RAD51
NCBI Official Synonym Symbols
RECA; BRCC5; FANCR; MRMV2; HRAD51; RAD51A; HsRad51; HsT16930
NCBI Protein Information
DNA repair protein RAD51 homolog 1
UniProt Protein Name
DNA repair protein RAD51 homolog 1
UniProt Gene Name
RAD51
UniProt Synonym Gene Names
RAD51A; RECA; HsRAD51; hRAD51
UniProt Entry Name
RAD51_HUMAN
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Product Notes
The Human RAD51 rad51 (Catalog #AAA331168) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA331168 ELISA Kit recognizes Human RAD51. It is sometimes possible for the material contained within the vial of "DNA Repair Protein RAD51 Homolog 1 (RAD51), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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