Standard Curve (Sample)
(Fig.1. Human DNA replication licensing factor MCM5 (MCM5) Standard Curve.)
Human DNA Replication Licensing Factor MCM5 (MCM5) ELISA Kit | MCM5 elisa kit
Human DNA Replication Licensing Factor MCM5 (MCM5) ELISA Kit
Gene Names
MCM5; CDC46; MGORS8; P1-CDC46
Reactivity
Human
Synonyms
DNA Replication Licensing Factor MCM5 (MCM5); N/A; Human DNA Replication Licensing Factor MCM5 (MCM5) ELISA Kit; MCM5; CDC46; MGC5315; P1-CDC46; DNA replication licensing factor; MCM5 minichromosome maintenance deficient 5; cell division cycle 46; minichromosome maintenance deficient 5 (cell division cycle 46); MCM5 elisa kit
Reactivity
Human
Specificity
Human DNA replication licensing factor MCM5 (MCM5) ELISA Kit has high sensitivity and excellent specificity for detection of Human MCM5. No significant cross-reactivity or interference between Human MCM5 and analogues was observed.
Sequence Length
734
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human DNA replication licensing factor MCM5 (MCM5) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human DNA replication licensing factor MCM5 (MCM5) Standard Curve.)
Related Product Information for MCM5 elisa kit
Description: This Human DNA replication licensing factor MCM5 (MCM5) ELISA Kit employs a two-site sandwich ELISA to quantitate MCM5 in samples. An antibody specific for MCM5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MCM5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MCM5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MCM5 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: MCM5 isa protein involved in the initiation of DNA replication. The encoded protein is a member of the MCM family of chromatin-binding proteins and can interact with at least two other members of this family. The encoded protein is upregulated in the transition from the G0 to G1/S phase of the cell cycle and may actively participate in cell cycle regulation.Depletion of ASF1 by RNA interference impeded DNA unwinding at replication sites, and similar defects arose from overproduction of new histone H3-H4 that compromised ASF1 function.ASF1, as a histone acceptor and donor, handles parental and new histones at the replication fork via an ASF1-(H3-H4)-MCM2-7 intermediate and thus provides a means to fine-tune replication fork progression and histone supply and demand.
Background: MCM5 isa protein involved in the initiation of DNA replication. The encoded protein is a member of the MCM family of chromatin-binding proteins and can interact with at least two other members of this family. The encoded protein is upregulated in the transition from the G0 to G1/S phase of the cell cycle and may actively participate in cell cycle regulation.Depletion of ASF1 by RNA interference impeded DNA unwinding at replication sites, and similar defects arose from overproduction of new histone H3-H4 that compromised ASF1 function.ASF1, as a histone acceptor and donor, handles parental and new histones at the replication fork via an ASF1-(H3-H4)-MCM2-7 intermediate and thus provides a means to fine-tune replication fork progression and histone supply and demand.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
DNA replication licensing factor MCM5
NCBI Official Synonym Full Names
minichromosome maintenance complex component 5
NCBI Official Symbol
MCM5
NCBI Official Synonym Symbols
CDC46; MGORS8; P1-CDC46
NCBI Protein Information
DNA replication licensing factor MCM5
UniProt Protein Name
DNA replication licensing factor MCM5
UniProt Gene Name
MCM5
UniProt Synonym Gene Names
CDC46
UniProt Entry Name
MCM5_HUMAN
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Product Notes
The Human MCM5 mcm5 (Catalog #AAA331840) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA331840 ELISA Kit recognizes Human MCM5. It is sometimes possible for the material contained within the vial of "DNA Replication Licensing Factor MCM5 (MCM5), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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