Standard Curve (Sample)
(Fig.1. Human E3 ubiquitin-protein ligase UBR1 (UBR1) Standard Curve.)
Human E3 Ubiquitin-Protein Ligase UBR1 (UBR1) ELISA Kit | UBR1 elisa kit
Human E3 Ubiquitin-Protein Ligase UBR1 (UBR1) ELISA Kit
Gene Names
UBR1; JBS
Reactivity
Human
Synonyms
E3 Ubiquitin-Protein Ligase UBR1 (UBR1); N/A; Human E3 Ubiquitin-Protein Ligase UBR1 (UBR1) ELISA Kit; UBR1; JBS; MGC142065; MGC142067; E3a ligase; ubiquitin ligase E3 alpha-I; ubiquitin-protein ligase E3-alpha; UBR1 elisa kit
Reactivity
Human
Specificity
Human E3 ubiquitin-protein ligase UBR1 (UBR1) ELISA Kit has high sensitivity and excellent specificity for detection of Human UBR1. No significant cross-reactivity or interference between Human UBR1 and analogues was observed.
Sequence Length
1749
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human E3 ubiquitin-protein ligase UBR1 (UBR1) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human E3 ubiquitin-protein ligase UBR1 (UBR1) Standard Curve.)
Related Product Information for UBR1 elisa kit
Description: This Human E3 ubiquitin-protein ligase UBR1 (UBR1) ELISA Kit employs a two-site sandwich ELISA to quantitate UBR1 in samples. An antibody specific for UBR1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any UBR1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for UBR1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of UBR1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: The mouse Ubr1 protein is a 1,757-residue, 200-kD protein that contains regions with sequence similarity to the 225-kD UBR1 protein of S. cerevisiae. There are apparent UBR1 homologs in other eukaryotes, thus defining a distinct family of proteins, the UBR family. The residues essential for substrate recognition by the yeast UBR1 protein are conserved in the mouse Ubr1 protein. Regions of similarity among the UBR family members include a putative zinc finger and RING-H2 finger, another zinc-binding domain. Northern blot analysis detected ubiquitous expression in human and adult mouse tissues, with highest levels in skeletal muscle and heart. In mouse embryos, in situ hybridization showed that Ubr1 expression is highest in the branchial arches and in the tail and limb buds.
Background: The mouse Ubr1 protein is a 1,757-residue, 200-kD protein that contains regions with sequence similarity to the 225-kD UBR1 protein of S. cerevisiae. There are apparent UBR1 homologs in other eukaryotes, thus defining a distinct family of proteins, the UBR family. The residues essential for substrate recognition by the yeast UBR1 protein are conserved in the mouse Ubr1 protein. Regions of similarity among the UBR family members include a putative zinc finger and RING-H2 finger, another zinc-binding domain. Northern blot analysis detected ubiquitous expression in human and adult mouse tissues, with highest levels in skeletal muscle and heart. In mouse embryos, in situ hybridization showed that Ubr1 expression is highest in the branchial arches and in the tail and limb buds.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
E3 ubiquitin-protein ligase UBR1
NCBI Official Synonym Full Names
ubiquitin protein ligase E3 component n-recognin 1
NCBI Official Symbol
UBR1
NCBI Official Synonym Symbols
JBS
NCBI Protein Information
E3 ubiquitin-protein ligase UBR1
UniProt Protein Name
E3 ubiquitin-protein ligase UBR1
UniProt Gene Name
UBR1
UniProt Entry Name
UBR1_HUMAN
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Product Notes
The Human UBR1 ubr1 (Catalog #AAA330319) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA330319 ELISA Kit recognizes Human UBR1. It is sometimes possible for the material contained within the vial of "E3 Ubiquitin-Protein Ligase UBR1 (UBR1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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