Human Engrailed Homeobox Protein 2 (EN2) ELISA Kit | EN2 elisa kit
Human Engrailed Homeobox Protein 2 (EN2) ELISA Kit
Gene Names
en; 153867_at; Apa; CG9015; DmelCG9015; En; EN; en1; Eng; Engr; Engrailed; Engrailed/Invected; EnR; Es; spa2; spt; V
Reactivity
Human
Synonyms
Engrailed Homeobox Protein 2 (EN2); N/A; Human Engrailed Homeobox Protein 2 (EN2) ELISA Kit; AUTS1; EN2 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of EN2. No significant cross-reactivity or interference between EN2 and analogues was observed.
Sequence Length
552
Assay Type
Double-antibody Sandwich
Samples
Tissue Homogenates And Other Biological Fluids
Detection Range
0.312-20ng/mL
Sensitivity
0.113ng/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level EN2 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level EN2 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Standard Curve (Sample)
Related Product Information for EN2 elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of EN2 in human tissue homogenates and other biological fluids.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to EN2. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to EN2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain EN2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of EN2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to EN2. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to EN2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain EN2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of EN2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Product Categories/Family for EN2 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
59,411 Da
NCBI Official Full Name
engrailed, isoform B
NCBI Official Synonym Full Names
engrailed
NCBI Official Symbol
en
NCBI Official Synonym Symbols
153867_at; Apa; CG9015; DmelCG9015; En; EN; en1; Eng; Engr; Engrailed; Engrailed/Invected; EnR; Es; spa2; spt; V
NCBI Protein Information
CG9015 gene product from transcript CG9015-RB
UniProt Protein Name
Segmentation polarity homeobox protein engrailed
UniProt Gene Name
en
UniProt Entry Name
HMEN_DROME
